Key features and details
- Rabbit polyclonal to MYO18A
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MYO18A antibody
See all MYO18A primary antibodies
DescriptionRabbit polyclonal to MYO18A
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Rat, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residue 2004-2054 of human MYO18A (NP_510880.2).
- RIPA whole lysates from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab81690 was affinity purified using an epitope specific to MYO18A immobilized on solid support.
Our Abpromise guarantee covers the use of ab81690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMyo18A/Myosin XVIIIA is an unconventional myosin that contains a lysine and glutamine (KE) rich domain and a PDZ domain. The KE and PDZ domains appear to direct subcellular localization of the Myo18A variants. It is a dimeric actin binding protein that may play a role in the stabilization and organization of the actin cytoskeleton. Recently a cell surface form of Myo18A has been identified as the receptor for surfactant protein A, a lipid associated lung collectin that mediates innate and adaptive immune responses in the lungs.
Cellular localizationIsoform 1: Endoplasmic reticulum Golgi intermediate compartment. Cytoplasm, cytoskeleton. Note: Colocalizes with actin. Isoform 2: Cytoplasm. Note: Lacks the PDZ domain. Diffusely localized in the cytoplasm.
- MAJN antibody
- Molecule associated with JAK3 N terminus antibody
- Myosin 18A antibody
Detection of human MYO18A by Western Blot of Immunoprecipitates.
Samples: RIPA whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
ab81690 used for IP at 3 µg/mg lysate (lane 2).
MYO18A was also immunoprecipitated by rabbit anti MYO18A (ab81689), which recognizes an upstream epitope (lane 1).
Lane 3 represents the control IgG.
For blotting immunoprecipitates, ab81689 was used at 1 µg/ml.
Detection: by Chemiluminescence with an exposure time of 1 second.
ab81690 has not yet been referenced specifically in any publications.