Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12551-13] to MYO19
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-MYO19 antibody [EPR12551-13]
See all MYO19 primary antibodies
DescriptionRabbit monoclonal [EPR12551-13] to MYO19
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC or IP
Species reactivityReacts with: Human
Recombinant fragment within Human MYO19. The exact sequence is proprietary.
Database link: Q96H55
- HeLa, K562 and 293T cell lysates; A549 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 109 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO19 is involved in mitochondrial motility.
Tissue specificityWidely expressed in multiple tissues and cell lines.
Sequence similaritiesContains 2 IQ domains.
Contains 1 myosin head-like domain.
Cellular localizationCytoplasm > cytoskeleton. Mitochondrion outer membrane. Probably associated with the outer membrane via its N-terminal mitochondrial targeting region.
- Information by UniProt
- FLJ22865 antibody
- myo19 antibody
- MYO19_HUMAN antibody
Immunofluorescent analysis of A549 cells labeling MYO19 with ab174286 at 1/50 (red). DAPI nuclear staining (blue).
All lanes : Anti-MYO19 antibody [EPR12551-13] (ab174286) at 1/1000 dilution
Lane 1 : HeLa cell line lysate
Lane 2 : A549 cell line lysate
Lane 3 : K562 cell line lysate
Lane 4 : 293T cell line lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 109 kDa
ab174286 has been referenced in 3 publications.
- Oeding SJ et al. Identification of Miro1 and Miro2 as mitochondrial receptors for myosin XIX. J Cell Sci 131:N/A (2018). PubMed: 30111583
- Shneyer BI et al. ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain. Sci Rep 7:11577 (2017). PubMed: 28912530
- Shneyer BI et al. Myo19 is an outer mitochondrial membrane motor and effector of starvation-induced filopodia. J Cell Sci 129:543-56 (2016). PubMed: 26659663