Product nameAnti-MYO19 antibody [EPR12551-13]
See all MYO19 primary antibodies
DescriptionRabbit monoclonal [EPR12551-13] to MYO19
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC or IP
Species reactivityReacts with: Human
Recombinant fragment within Human MYO19. The exact sequence is proprietary.
Database link: Q96H55
- HeLa, K562 and 293T cell lysates; A549 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 109 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO19 is involved in mitochondrial motility.
Tissue specificityWidely expressed in multiple tissues and cell lines.
Sequence similaritiesContains 2 IQ domains.
Contains 1 myosin head-like domain.
Cellular localizationCytoplasm > cytoskeleton. Mitochondrion outer membrane. Probably associated with the outer membrane via its N-terminal mitochondrial targeting region.
- Information by UniProt
- FLJ22865 antibody
- myo19 antibody
- MYO19_HUMAN antibody
Immunofluorescent analysis of A549 cells labeling MYO19 with ab174286 at 1/50 (red). DAPI nuclear staining (blue).
All lanes : Anti-MYO19 antibody [EPR12551-13] (ab174286) at 1/1000 dilution
Lane 1 : HeLa cell line lysate
Lane 2 : A549 cell line lysate
Lane 3 : K562 cell line lysate
Lane 4 : 293T cell line lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 109 kDa
This product has been referenced in:
- Shneyer BI et al. ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain. Sci Rep 7:11577 (2017). Read more (PubMed: 28912530) »
- Shneyer BI et al. Myo19 is an outer mitochondrial membrane motor and effector of starvation-induced filopodia. J Cell Sci 129:543-56 (2016). Read more (PubMed: 26659663) »