Overview

  • Product name

    Anti-MYO19 antibody [EPR12551-13]
    See all MYO19 primary antibodies
  • Description

    Rabbit monoclonal [EPR12551-13] to MYO19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human MYO19. The exact sequence is proprietary.
    Database link: Q96H55

  • Positive control

    • HeLa, K562 and 293T cell lysates; A549 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12551-13
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 109 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC or IP.
  • Target

    • Function

      Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO19 is involved in mitochondrial motility.
    • Tissue specificity

      Widely expressed in multiple tissues and cell lines.
    • Sequence similarities

      Contains 2 IQ domains.
      Contains 1 myosin head-like domain.
    • Cellular localization

      Cytoplasm > cytoskeleton. Mitochondrion outer membrane. Probably associated with the outer membrane via its N-terminal mitochondrial targeting region.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ22865 antibody
      • myo19 antibody
      • MYO19_HUMAN antibody
      • Myohd1 antibody
      • Myosin head domain containing 1 antibody
      • Myosin head domain-containing protein 1 antibody
      • myosin XIX antibody
      • Myosin-XIX antibody
      • unconventional myosin-XIX antibody
      see all

    Images

    • Immunofluorescent analysis of A549 cells labeling MYO19 with ab174286 at 1/50  (red). DAPI nuclear staining (blue).

    • All lanes : Anti-MYO19 antibody [EPR12551-13] (ab174286) at 1/1000 dilution

      Lane 1 : HeLa cell line lysate
      Lane 2 : A549 cell line lysate
      Lane 3 : K562 cell line lysate
      Lane 4 : 293T cell line lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution

      Developed using the ECL technique.

      Predicted band size: 109 kDa

    References

    This product has been referenced in:

    • Shneyer BI  et al. ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain. Sci Rep 7:11577 (2017). Read more (PubMed: 28912530) »
    • Shneyer BI  et al. Myo19 is an outer mitochondrial membrane motor and effector of starvation-induced filopodia. J Cell Sci 129:543-56 (2016). Read more (PubMed: 26659663) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174286.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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