Recombinant
RabMAb

Recombinant Anti-MYO1B antibody [EPR16223] (ab194356)

Overview

  • Product name

    Anti-MYO1B antibody [EPR16223]
    See all MYO1B primary antibodies
  • Description

    Rabbit monoclonal [EPR16223] to MYO1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human MYO1B aa 900 to the C-terminus. The exact sequence is proprietary.
    Database link: O43795

  • Positive control

    • Human fetal kidney lysate; A431, C6 and 293 cell lysate; Mouse and rat heart lysates; Human pancreas and rat liver tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16223
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab194356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).

Target

Images

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MYO1B with ab194356 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin.

  • All lanes : Anti-MYO1B antibody [EPR16223] (ab194356) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : 293 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 132 kDa
    Observed band size: 132 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MYO1B with ab194356 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin.

  • All lanes : Anti-MYO1B antibody [EPR16223] (ab194356) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate
    Lane 3 : C6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 132 kDa
    Observed band size: 132 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Yu Y  et al. Arginase-II activates mTORC1 through myosin-1b in vascular cell senescence and apoptosis. Cell Death Dis 9:313 (2018). Read more (PubMed: 29472548) »
  • Baldwin RM  et al. Identification of the PRMT1v1 and PRMT1v2 specific interactomes by quantitative mass spectrometry in breast cancer cells. Proteomics 15:2187-97 (2015). Read more (PubMed: 25690678) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Ins-1)
Permeabilization
Yes - saponin
Specification
Ins-1
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 13 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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