Overview

  • Product name

    Anti-MYO1D antibody
  • Description

    Mouse polyclonal to MYO1D
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length human MYO1D protein (isoform 2: AAH30602.1).

  • Positive control

    • MYO1D transfected 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight for isoform 2: 50 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Myosins are actin based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments.
    • Database links

    • Alternative names

      • KIAA0727 antibody
      • Myosin I gamma antibody
      • Myosin Id antibody
      • MYR4 antibody

    Images

    • All lanes : Anti-MYO1D antibody (ab70204) at 1/500 dilution

      Lane 1 : MYO1D transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 50 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Saydmohammed M  et al. Vertebrate myosin 1d regulates left-right organizer morphogenesis and laterality. Nat Commun 9:3381 (2018). Read more (PubMed: 30139971) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70204.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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