Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab177248 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab177248 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 118 kDa).
An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide.
- Myosin-1H antibody
- Unconventional myosin-Ih antibody
Anti-MYO1H antibody (ab177248) at 0.1 µg/ml + NIH-3T3 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 118 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour.
ab177248 has not yet been referenced specifically in any publications.