Key features and details
- Goat polyclonal to MYO1H - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MYO1H antibody - C-terminal
DescriptionGoat polyclonal to MYO1H - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- NIH3T3 cell lysate; Human kidney tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 119 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
- Myosin-1H antibody
- Unconventional myosin-Ih antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling MYO1H with ab219505 at 5 μg/ml.
Anti-MYO1H antibody - C-terminal (ab219505) at 0.1 µg/ml + NIH3T3 cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 119 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219505 has not yet been referenced specifically in any publications.