Overview

  • Product name

  • Description

    Rabbit polyclonal to MYO5B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human MYO5B.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 214 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.
  • Involvement in disease

    Diarrhea 2, with microvillus atrophy
  • Sequence similarities

    Contains 1 dilute domain.
    Contains 6 IQ domains.
    Contains 1 myosin head-like domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA1119 antibody
    • Myo5b antibody
    • MYO5B_HUMAN antibody
    • Myosin Vb antibody
    • Unconventional myosin-Vb antibody
    see all

Images

  • ab58766 staining MYO5B in mouse hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA + 0.1% saponin) for 20 hours at 4°C. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References

ab58766 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Hippocampal neurons)
Specification
Hippocampal neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 17 2014

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (7% SDS)
Sample
Mouse Tissue lysate - other (brain)
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Mar 17 2014

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