Anti-MYO6 antibody (ab230478)
Key features and details
- Rabbit polyclonal to MYO6
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MYO6 antibody
See all MYO6 primary antibodies -
Description
Rabbit polyclonal to MYO6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Pig -
Immunogen
Synthetic peptide corresponding to Human MYO6 (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q9UM54 -
Positive control
- IHC-P: Human pancreas and kidney tissues. WB: MCF7 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/100.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 150 kDa.
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Notes |
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IHC-P
1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 150 kDa. |
Target
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Function
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells. -
Tissue specificity
Expressed in most tissues examined including heart, brain, placenta, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in brain, pancreas, testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2, containing the small insert, and isoform 4, containing neither insert, are expressed in unpolarized epithelial cells. -
Involvement in disease
Defects in MYO6 are the cause of deafness autosomal dominant type 22 (DFNA22) [MIM:606346]. DFNA22 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA22 is progressive and postlingual, with onset during childhood. By the age of approximately 50 years, affected individuals invariably have profound sensorineural deafness.
Defects in MYO6 are the cause of deafness autosomal recessive type 37 (DFNB37) [MIM:607821].
Defects in MYO6 are the cause of deafness sensorineural with hypertrophic cardiomyopathy (DFNHCM) [MIM:606346]. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Domain
Divided into three regions: a N-terminal motor (head) domain, followed by a neck domain consisting of a calmodulin-binding linker domain and a single IQ motif, and a C-terminal tail region with a coiled-coil and a unique globular domain required for interaction with other proteins. -
Post-translational
modificationsPhosphorylation in the motor domain, induced by EGF, results in translocation of MYO6 from the cell surface to membrane ruffles and affects F-actin dynamics. Phosphorylated in vitro by p21-activated kinase (PAK). -
Cellular localization
Cytoplasmic vesicle > clathrin-coated vesicle membrane; Cytoplasmic vesicle > clathrin-coated vesicle membrane. Cell projection > ruffle membrane and Golgi apparatus > trans-Golgi network membrane. Golgi apparatus. Nucleus. Cytoplasm > perinuclear region. Membrane > clathrin-coated pit. Cell projection > ruffle membrane. Also present in endocyctic vesicles, and membrane ruffles. Translocates from membrane ruffles, endocytic vesicles and cytoplasm to Golgi apparatus, perinuclear membrane and nucleus through induction by p53 and p53-induced DNA damage. Recruited into membrane ruffles from cell surface by EGF-stimulation. Colocalizes with DAB2 in clathrin-coated pits/vesicles. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 4646 Human
- Entrez Gene: 17920 Mouse
- Entrez Gene: 315840 Rat
- Omim: 600970 Human
- SwissProt: Q9UM54 Human
- SwissProt: Q64331 Mouse
- Unigene: 149387 Human
- Unigene: 4040 Mouse
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Alternative names
- Deafness autosomal recessive 37 antibody
- DFNA 22 antibody
- DFNA22 antibody
see all
Images
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Anti-MYO6 antibody (ab230478) at 1/500 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 150 kDa -
Formalin-fixed, paraffin-embedded human kidney tissue stained for MYO6 using ab230478 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for MYO6 using ab230478 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab230478 has been referenced in 3 publications.
- Gu J et al. LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis. World J Surg Oncol 19:281 (2021). PubMed: 34535152
- Zhang L et al. Circ_0026416 downregulation blocks the development of colorectal cancer through depleting MYO6 expression by enriching miR-545-3p. World J Surg Oncol 19:299 (2021). PubMed: 34645476
- Duan Q et al. Long noncoding RNA UCA1 promotes cell growth, migration, and invasion by targeting miR-143-3p in oral squamous cell carcinoma. Cancer Med 9:3115-3129 (2020). PubMed: 32130788