Overview

  • Product name
    Anti-MYO9B antibody
  • Description
    Goat polyclonal to MYO9B
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-KNDFEPVKQSKAQ

    , corresponding to internal sequence amino acids 1605-1617 of Human MYO9B

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human Liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:2,000.
    WB: Use at a concentration of 2 - 6 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 244 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance
      Myosin IX belongs to the family of unconventional myosins, distinct from the classical myosins I and myosins II. Myosin IX, thought to be involved in signal transduction and leukocyte differentiation, includes several isoforms. The rat isoforms are myosin IX/Myr5 (230 kDa) and Myr7 (300 kDa), and the human isoforms are myosin IXa (MYO9A) and myosin IXb (MYO9B). The isoforms of myosin IX have similar overall domain structure and are expressed in many tissues and cell types, although they have distinct tissue expression patterns. The subcellular localization of class IX myosins appears to be partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. The expression level of myosins IX may vary during development and differentiation.
    • Cellular localization
      Cytoplasm; cell cortex. Cytoplasm; perinuclear region. Note=In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region.
    • Database links
      • Alternative names
        • CELIAC 4 antibody
        • Celiac disease 4 antibody
        • CELIAC4 antibody
        • MYO 9B antibody
        • Myosin 9B antibody
        • Myosin IX antibody
        • Myosin IXB antibody
        • Myosin9B antibody
        • MyosinIX antibody
        • MyosinIXB antibody
        • MYR 5 antibody
        • MYR5 antibody
        • Unconventional myosin 9b antibody
        see all

      Images

      • Western blot - Anti-MYO9B antibody (ab52026)
        Western blot - Anti-MYO9B antibody (ab52026)
        Anti-MYO9B antibody (ab52026) at 2 µg/ml + Human Liver lysate (35µg protein in RIPA buffer).

        Predicted band size: 244 kDa
        Observed band size: 200 kDa (why is the actual band size different from the predicted?)



        Primary incubation was 1 hour. Detected by chemiluminescence.

      References

      ab52026 has not yet been referenced specifically in any publications.

      Publishing research using ab52026? Please let us know so that we can cite the reference in this datasheet.

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