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    myo9b-antibody-ab52026.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Anti-MYO9B antibody (ab52026)

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Western blot - Anti-MYO9B antibody (ab52026)

    Key features and details

    • Goat polyclonal to MYO9B
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    View more associated products

    Overview

    • Product name

      Anti-MYO9B antibody
      See all MYO9B primary antibodies
    • Description

      Goat polyclonal to MYO9B
    • Host species

      Goat
    • Tested Applications & Species

      Application Species
      Flow Cyt (Intra)
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      C-KNDFEPVKQSKAQ

      , corresponding to internal sequence amino acids 1605-1617 of Human MYO9B
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Human Liver lysate.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Motor Proteins
      • Myosin

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab52026 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt (Intra)
    Human
    Application Abreviews Notes
    WB
    Notes
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:2,000.
    WB: Use at a concentration of 2 - 6 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 244 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Myosin IX belongs to the family of unconventional myosins, distinct from the classical myosins I and myosins II. Myosin IX, thought to be involved in signal transduction and leukocyte differentiation, includes several isoforms. The rat isoforms are myosin IX/Myr5 (230 kDa) and Myr7 (300 kDa), and the human isoforms are myosin IXa (MYO9A) and myosin IXb (MYO9B). The isoforms of myosin IX have similar overall domain structure and are expressed in many tissues and cell types, although they have distinct tissue expression patterns. The subcellular localization of class IX myosins appears to be partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. The expression level of myosins IX may vary during development and differentiation.
    • Cellular localization

      Cytoplasm; cell cortex. Cytoplasm; perinuclear region. Note=In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region.
    • Database links

      • Entrez Gene: 4650 Human
      • Omim: 602129 Human
      • SwissProt: Q13459 Human
      • Unigene: 159629 Human
      • Alternative names

        • CELIAC 4 antibody
        • Celiac disease 4 antibody
        • CELIAC4 antibody
        • MYO 9B antibody
        • Myosin 9B antibody
        • Myosin IX antibody
        • Myosin IXB antibody
        • Myosin9B antibody
        • MyosinIX antibody
        • MyosinIXB antibody
        • MYR 5 antibody
        • MYR5 antibody
        • Unconventional myosin 9b antibody
        see all

      Images

      • Western blot - Anti-MYO9B antibody (ab52026)
        Western blot - Anti-MYO9B antibody (ab52026)
        Anti-MYO9B antibody (ab52026) at 2 µg/ml + Human Liver lysate (35µg protein in RIPA buffer).

        Predicted band size: 244 kDa
        Observed band size: 200 kDa
        why is the actual band size different from the predicted?



        Primary incubation was 1 hour. Detected by chemiluminescence.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab52026? Please let us know so that we can cite the reference in this datasheet.

      ab52026 has not yet been referenced specifically in any publications.

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