Anti-MYO9B antibody (ab52026)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-MYO9B antibody
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DescriptionGoat polyclonal to MYO9B
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Host speciesGoat
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide:
C-KNDFEPVKQSKAQ
, corresponding to internal sequence amino acids 1605-1617 of Human MYO9B -
Positive control
- Human Liver lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab52026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| WB |
Application notes
Peptide ELISA: Antibody detection limit dilution 1:2,000.
WB: Use at a concentration of 2 - 6 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 244 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 2 - 6 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 244 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceMyosin IX belongs to the family of unconventional myosins, distinct from the classical myosins I and myosins II. Myosin IX, thought to be involved in signal transduction and leukocyte differentiation, includes several isoforms. The rat isoforms are myosin IX/Myr5 (230 kDa) and Myr7 (300 kDa), and the human isoforms are myosin IXa (MYO9A) and myosin IXb (MYO9B). The isoforms of myosin IX have similar overall domain structure and are expressed in many tissues and cell types, although they have distinct tissue expression patterns. The subcellular localization of class IX myosins appears to be partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. The expression level of myosins IX may vary during development and differentiation.
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Cellular localizationCytoplasm; cell cortex. Cytoplasm; perinuclear region. Note=In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region.
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Database links
- Entrez Gene: 4650 Human
- Omim: 602129 Human
- SwissProt: Q13459 Human
- Unigene: 159629 Human
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Alternative names
- CELIAC 4 antibody
- Celiac disease 4 antibody
- CELIAC4 antibody
see all
Images
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Western blot - Anti-MYO9B antibody (ab52026)Anti-MYO9B antibody (ab52026) at 2 µg/ml + Human Liver lysate (35µg protein in RIPA buffer).
Predicted band size: 244 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
Protocols
References
ab52026 has not yet been referenced specifically in any publications.
