Anti-MYO9B antibody (ab52026)
Key features and details
- Goat polyclonal to MYO9B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MYO9B antibody
See all MYO9B primary antibodies -
Description
Goat polyclonal to MYO9B -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
C-KNDFEPVKQSKAQ
, corresponding to internal sequence amino acids 1605-1617 of Human MYO9B -
Positive control
- Human Liver lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
| Notes |
|---|
Application notes
Peptide ELISA: Antibody detection limit dilution 1:2,000.
WB: Use at a concentration of 2 - 6 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 244 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 2 - 6 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 244 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions (PubMed:9490638). Also acts as a GTPase activator for RHOA (PubMed:9490638, PubMed:26529257). Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). -
Tissue specificity
Detected in peripheral blood leukocytes (at protein level) (PubMed:9490638). Expressed predominantly in peripheral blood leukocytes and at lower levels, in thymus, spleen, testis, prostate, ovary, brain, small intestine and lung. -
Involvement in disease
Celiac disease 4 -
Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
Contains 4 IQ domains.
Contains 1 myosin motor domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-associating domain.
Contains 1 Rho-GAP domain. -
Cellular localization
Cytoplasm, cell cortex. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 4650 Human
- Omim: 602129 Human
- SwissProt: Q13459 Human
- Unigene: 123198 Human
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Alternative names
- CELIAC 4 antibody
- Celiac disease 4 antibody
- CELIAC4 antibody
see all
Images
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Western blot - Anti-MYO9B antibody (ab52026)Anti-MYO9B antibody (ab52026) at 2 µg/ml + Human Liver lysate (35µg protein in RIPA buffer).
Predicted band size: 244 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (0)
ab52026 has not yet been referenced specifically in any publications.
