Overview

  • Product name

  • Description

    Rabbit polyclonal to Myocardin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal domain of Human Myocardin.

Properties

Applications

Our Abpromise guarantee covers the use of ab106346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 102 kDa.
IHC-Fr 1/50 - 1/500.

Target

  • Function

    Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
  • Tissue specificity

    Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
  • Sequence similarities

    Contains 3 RPEL repeats.
    Contains 1 SAP domain.
  • Domain

    The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • MYCD antibody
    • MYCD_HUMAN antibody
    • Myocardin antibody
    • Myocd antibody

References

This product has been referenced in:

  • Luo Y  et al. MiR-466h-5p induces expression of myocardin with complementary promoter sequences. Biochem Biophys Res Commun 514:187-193 (2019). Read more (PubMed: 31029421) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (retina)
Antigen retrieval step
None
Permeabilization
Yes - 1% Triton
Specification
retina
Blocking step
normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 09 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (pulmonary artery smooth muscle cell)
Loading amount
14 µg
Specification
pulmonary artery smooth muscle cell
Treatment
both treated and not treated by anti-mir145 30nM for 4 days
Gel Running Conditions
Non-reduced Denaturing (10% BisTris Gel)
Blocking step
Licor Blocking buffer as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 15 2011

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