Product nameAnti-Myocardin antibody
See all Myocardin primary antibodies
DescriptionRabbit polyclonal to Myocardin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Chicken, Human
Predicted to work with: Mouse, Rat, Rabbit, Pig, Macaque monkey, Gorilla
Synthetic peptide corresponding to Human Myocardin aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Small Intestine tissue lysate and Caco2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab107301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 101 kDa).|
FunctionTranscriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
Tissue specificityExpressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
Sequence similaritiesContains 3 RPEL repeats.
Contains 1 SAP domain.
DomainThe C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.
- Information by UniProt
- MYCD antibody
- MYCD_HUMAN antibody
- Myocardin antibody
- Myocd antibody
All lanes : Anti-Myocardin antibody (ab107301) at 1 µg/ml
Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 101 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa, 26 kDa, 48 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds