Product nameAnti-MyoD1 antibody [5.2F]
See all MyoD1 primary antibodies
DescriptionMouse monoclonal [5.2F] to MyoD1
SpecificityThis antibody does not cross react with Myogenin, Myf5, or Myf6.
Tested applicationsSuitable for: ICC/IF, Sandwich ELISA, ELISA, IP, IHC-P, IHC-Fr, Electron Microscopy, WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Predicted to work with: Sheep, Cow, Pig
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
sELISA pair antibody
Our Abpromise guarantee covers the use of ab16148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MyoD1 (ab64159). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to MyoD1 (ab64159) as Detection.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at 2 µg/mg of lysate.|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
|IHC-Fr||Use a concentration of 2 - 4 µg/ml.|
|Electron Microscopy||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.|
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
- Information by UniProt
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
All lanes : Anti-MyoD1 antibody [5.2F] (ab16148) at 1/1000 dilution
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Mouse liver tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 20 seconds
ab16148 staining MyoD1 in rat differentiated skeletal muscle cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde and then blocked using 2% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 8 hours. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical staining using ab16148 at 2ug/ml on formalin fixed, paraffin embedded samples of human rhabdomyosarcoma.
This product has been referenced in:
- Miersch C et al. Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation. BMC Cell Biol 19:2 (2018). Flow Cyt . Read more (PubMed: 29523096) »
- Bala U et al. Defects in nerve conduction velocity and different muscle fibre-type specificity contribute to muscle weakness in Ts1Cje Down syndrome mouse model. PLoS One 13:e0197711 (2018). Read more (PubMed: 29795634) »