Key features and details
- Mouse monoclonal [5.8A] to MyoD1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MyoD1 antibody [5.8A]
See all MyoD1 primary antibodies
DescriptionMouse monoclonal [5.8A] to MyoD1
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant full length protein corresponding to Mouse MyoD1.
MELLSPPLRDIDLTGPDGSLCSFETADDFYDDPCFDSPDLRFFEDLDPRL VHMGALLKPEEHAHFPTAVHPGPGAREDEHVRAPSGHHQAGRCLLWACKA CKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEIL RNAIRYIEGLQALLRDQDAAPPGAAAFYAPGPLPPGRGSEHYSGDSDASS PRSNCSDGMMDYSGPPSGPRRQNGYDTAYYSEAARESRPGKSAAVSSLDC LSSIVERISTDSPAAPALLLADAPPESPPGPPEGASLSDTEQGTQTPSPD AAPQCPAGSNPNAIYQVL
Database link: P10085
EpitopeThe epitope of ab233941 maps between amino acid 180-189 in the C terminal of mouse MyoD1 protein.
- IHC-P: Human rhabdomyosarcoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from Bioreactor Concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab233941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at room temperature.
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
- Information by UniProt
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233941 has been referenced in 1 publication.
- Stuelsatz P et al. Down-regulation of MyoD by calpain 3 promotes generation of reserve cells in C2C12 myoblasts. J Biol Chem 285:12670-83 (2010). PubMed: 20139084