Key features and details
- Mouse monoclonal [5.8A] to MyoD1 - BSA free
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG1
Product nameAnti-MyoD1 antibody [5.8A] - BSA free
See all MyoD1 primary antibodies
DescriptionMouse monoclonal [5.8A] to MyoD1 - BSA free
Specificityab212662 does not cross react with myogenin, Myf5, or Myf6.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Recombinant full length protein corresponding to Mouse MyoD1.
Database link: P10085
EpitopeThe epitope of ab212662 maps between amino acid 180-189 in the C terminal of mouse MyoD1 protein.
- Human rhabdomyosarcoma tissue
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from bioreactor concentrate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab212662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
for 30 minutes at room temperature.
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
- Information by UniProt
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
ab212662 has not yet been referenced specifically in any publications.