Anti-MyoD1 antibody [5.8A] - BSA free (ab212662)

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Overview

  • Product name
    Anti-MyoD1 antibody [5.8A] - BSA free
    See all MyoD1 primary antibodies
  • Description
    Mouse monoclonal [5.8A] to MyoD1 - BSA free
  • Host species
    Mouse
  • Specificity
    ab212662 does not cross react with myogenin, Myf5, or Myf6.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse MyoD1.
    Database link: P10085

  • Epitope
    The epitope of ab212662 maps between amino acid 180-189 in the C terminal of mouse MyoD1 protein.
  • Positive control
    • Human rhabdomyosarcoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab212662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

for 30 minutes at room temperature.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

Final volume 100 µL.

Target

  • Function
    Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
    Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • bHLHc1 antibody
    • Class C basic helix-loop-helix protein 1 antibody
    • MYF 3 antibody
    • Myf-3 antibody
    • MYF3 antibody
    • Myoblast determination protein 1 antibody
    • Myod 1 antibody
    • MYOD antibody
    • MYOD1 antibody
    • MYOD1_HUMAN antibody
    • Myogenic differentiation 1 antibody
    • Myogenic factor 3 antibody
    • Myogenic factor MYF 3 antibody
    • Myogenin D1 antibody
    • PUM antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.8A] - BSA free (ab212662)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.8A] - BSA free (ab212662)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Rhabdomyosarcoma tissue section labeling MyoD1 with ab212662 at 1 µg/mL.

References

ab212662 has not yet been referenced specifically in any publications.

Publishing research using ab212662? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyoD1 antibody [5.8A] - BSA free

 Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Hamster Tissue sections (Muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0
Permeabilization
No
Specification
Muscle
Blocking step
BSA + Milk + Serum as blocking agent for 20 minute(s) · Concentration: 2.0% · Temperature: 27°C
Fixative
Formaldehyde
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Username

Dr. Marcelo Pelajo Machado

Verified customer

Submitted Jul 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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