Anti-MyoD1 antibody [5.8A] - BSA free (ab212662)
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Overview
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Product name
Anti-MyoD1 antibody [5.8A] - BSA free
See all MyoD1 primary antibodies -
Description
Mouse monoclonal [5.8A] to MyoD1 - BSA free -
Host species
Mouse -
Specificity
ab212662 does not cross react with myogenin, Myf5, or Myf6. -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human -
Immunogen
Recombinant full length protein corresponding to Mouse MyoD1.
Database link: P10085 -
Epitope
The epitope of ab212662 maps between amino acid 180-189 in the C terminal of mouse MyoD1 protein. -
Positive control
- Human rhabdomyosarcoma tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
5.8A -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab212662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. for 30 minutes at room temperature. |
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ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
Flow Cyt | Use 0.5-1µg for 106 cells. Final volume 100 µL. |
Target
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Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 374048 Chicken
- Entrez Gene: 4654 Human
- Entrez Gene: 17927 Mouse
- Entrez Gene: 337868 Rat
- Omim: 159970 Human
- SwissProt: P16075 Chicken
- SwissProt: P15172 Human
- SwissProt: P10085 Mouse
see all -
Alternative names
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
Images
Protocols
Datasheets and documents
References
ab212662 has not yet been referenced specifically in any publications.