Anti-MyoD1 antibody [5.8A] - BSA free (ab212662)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.8A] - BSA free (ab212662)

    Key features and details

    • Mouse monoclonal [5.8A] to MyoD1 - BSA free
    • Suitable for: IHC-P
    • Reacts with: Mouse, Rat, Chicken, Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-MyoD1 antibody [5.8A] - BSA free
      See all MyoD1 primary antibodies

    • Description

      Mouse monoclonal [5.8A] to MyoD1 - BSA free

    • Host species

      Mouse

    • Specificity

      ab212662 does not cross react with myogenin, Myf5, or Myf6.

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Chicken, Human

    • Immunogen

      Recombinant full length protein corresponding to Mouse MyoD1.
      Database link: P10085

    • Epitope

      The epitope of ab212662 maps between amino acid 180-189 in the C terminal of mouse MyoD1 protein.

    • Positive control

      • Human rhabdomyosarcoma tissue

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab212662 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    for 30 minutes at room temperature.

    Target

    • Function

      Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.

    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.

    • Post-translational
      modifications

      Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
      Ubiquitinated on the N-terminus; which is required for proteasomal degradation.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • bHLHc1 antibody
      • Class C basic helix-loop-helix protein 1 antibody
      • MYF 3 antibody
      • Myf-3 antibody
      • MYF3 antibody
      • Myoblast determination protein 1 antibody
      • Myod 1 antibody
      • MYOD antibody
      • MYOD1 antibody
      • MYOD1_HUMAN antibody
      • Myogenic differentiation 1 antibody
      • Myogenic factor 3 antibody
      • Myogenic factor MYF 3 antibody
      • Myogenin D1 antibody
      • PUM antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.8A] - BSA free (ab212662)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.8A] - BSA free (ab212662)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Rhabdomyosarcoma tissue section labeling MyoD1 with ab212662 at 1 µg/mL.

    References (0)

    Publishing research using ab212662? Please let us know so that we can cite the reference in this datasheet.

    ab212662 has not yet been referenced specifically in any publications.

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyoD1 antibody [5.8A] - BSA free

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Hamster Tissue sections (Muscle)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0
    Permeabilization
    No
    Specification
    Muscle
    Blocking step
    BSA + Milk + Serum as blocking agent for 20 minute(s) · Concentration: 2.0% · Temperature: 27°C
    Fixative
    Formaldehyde
    Read More

    Dr. Marcelo Pelajo Machado

    Verified customer

    Submitted Jul 21 2017

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