Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Hamster Tissue sections (Muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0
Permeabilization
No
Specification
Muscle
Blocking step
BSA + Milk + Serum as blocking agent for 20 minute(s) · Concentration: 2.0% · Temperature: 27°C
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/100
Secondary antibody
Dilution
1/750
Name
Non-Abcam antibody was used: ThermoFischer A-11001 - Goat anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Specimen: hamster muscle sections
Fixative: Formalin in Millonig buffer (48 hours)
Antigen retrieval in Pascal(R) chamber
Counterstainings: Evans blue (Red) and DAPI (Blue)
Image acquisition: Zeiss LSM 710 confocal microscope
Obs: This antibody did not work in this system, as well as in our positive controls (chicken embryo muscle sections and cell culture)
Abcam response
The end user has tested this antibody under Abtrial offer (https://www.abcam.com/content/abtrial-program-test-risk-free).
Dr. Marcelo Pelajo Machado
Verified customer
Submitted Jul 21 2017