Key features and details
- Rabbit polyclonal to MyoD1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MyoD1 antibody
See all MyoD1 primary antibodies
DescriptionRabbit polyclonal to MyoD1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods.
|WB||1/100 - 1/1000. Predicted molecular weight: 34 kDa.|
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
- Information by UniProt
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
Immunohistochemical analysis of formalin-fixed and paraffin embedded embryonic rhabdomyoma tissue labeling MyoD1using ab203333 at 1/200, followed by secondary detection and DAB staining.
All lanes : Anti-MyoD1 antibody (ab203383) at 1/300 dilution
Lane 1 : Rat muscle lysate
Lane 2 : Mouse muscle lysate
Predicted band size: 34 kDa
ab203383 has been referenced in 6 publications.
- Zhu M et al. miR-217-5p regulates myogenesis in skeletal muscle stem cells by targeting FGFR2. Mol Med Rep 22:850-858 (2020). PubMed: 32626929
- Li D et al. Long non-coding RNA MALAT1 promotes cardiac remodeling in hypertensive rats by inhibiting the transcription of MyoD. Aging (Albany NY) 11:8792-8809 (2019). PubMed: 31619581
- Zhao Z et al. miR-181b-5p May Regulate Muscle Growth in Tilapia by Targeting Myostatin b. Front Endocrinol (Lausanne) 10:812 (2019). PubMed: 31849840
- Hsieh PL et al. Recruitment and therapeutic application of macrophages in skeletal muscles after hind limb ischemia. J Vasc Surg 67:1908-1920.e1 (2018). PubMed: 29273298
- Rybalko V et al. Therapeutic potential of adipose-derived stem cells and macrophages for ischemic skeletal muscle repair. Regen Med 12:153-167 (2017). PubMed: 28244825
- Parada C et al. Disruption of the ERK/MAPK pathway in neural crest cells as a potential cause of Pierre Robin sequence. Development 142:3734-45 (2015). PubMed: 26395480