Product nameAnti-MyoD1 antibody
See all MyoD1 primary antibodies
DescriptionRabbit polyclonal to MyoD1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- IHC-P: Embryonic rhabdomyoma. WB: mouse and rat muscle lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods.
|WB||1/100 - 1/1000. Predicted molecular weight: 34 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
- Information by UniProt
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
Immunohistochemical analysis of formalin-fixed and paraffin embedded embryonic rhabdomyoma tissue labeling MyoD1using ab203333 at 1/200, followed by secondary detection and DAB staining.
All lanes : Anti-MyoD1 antibody (ab203383) at 1/300 dilution
Lane 1 : Rat muscle lysate
Lane 2 : Mouse muscle lysate
Predicted band size: 34 kDa
This product has been referenced in:
- Rybalko V et al. Therapeutic potential of adipose-derived stem cells and macrophages for ischemic skeletal muscle repair. Regen Med 12:153-167 (2017). Read more (PubMed: 28244825) »
- Parada C et al. Disruption of the ERK/MAPK pathway in neural crest cells as a potential cause of Pierre Robin sequence. Development 142:3734-45 (2015). Read more (PubMed: 26395480) »