Key features and details
- Rabbit polyclonal to MyoD1
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MyoD1 antibody
See all MyoD1 primary antibodies
DescriptionRabbit polyclonal to MyoD1
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Pig, Rhesus monkey
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
sELISA pair antibody
Our Abpromise guarantee covers the use of ab64159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5.2F] to MyoD1 (ab16148). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [5.2F] to MyoD1 (ab16148) as Capture.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).|
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
- Information by UniProt
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
All lanes : Anti-MyoD1 antibody (ab64159) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate at 20 µg
Lane 2 : Rh30 Whole Cell Lysate at 5 µg
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa (possible post-translational modification)
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab64159 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab64159 detects a band at 45 kDa, while this differs to its predicted molecular weight of 34kDa, the banding pattern observed is consistent with what has been described in the literature PMID:19352326.
ab64159 has been referenced in 14 publications.
- Sato T et al. Core Transcription Factors Promote Induction of PAX3-Positive Skeletal Muscle Stem Cells. Stem Cell Reports 13:352-365 (2019). PubMed: 31353225
- Zhang P et al. Salidroside Inhibits Myogenesis by Modulating p-Smad3-Induced Myf5 Transcription. Front Pharmacol 9:209 (2018). PubMed: 29593538
- Mota R et al. Increasing Cardiomyocyte Atrogin-1 Reduces Aging-Associated Fibrosis and Regulates Remodeling in Vivo. Am J Pathol 188:1676-1692 (2018). PubMed: 29758183
- Chen HS et al. Benzyl butyl phthalate decreases myogenic differentiation of endometrial mesenchymal stem/stromal cells through miR-137-mediated regulation of PITX2. Sci Rep 7:186 (2017). IF . PubMed: 28298639
- Zeng X et al. Acylated and unacylated ghrelin inhibit atrophy in myotubes co-cultured with colon carcinoma cells. Oncotarget 8:72872-72885 (2017). PubMed: 29069832