Product nameAnti-MyoD1 antibody
See all MyoD1 primary antibodies
DescriptionRabbit polyclonal to MyoD1
Tested applicationsSuitable for: Sandwich ELISA, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Pig, Rhesus monkey
- This antibody gave a positive signal in Mouse Skeletal Muscle tissue lysate as well as the Human Rhabdomyosarcoma Cell Line Rh30.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
sELISA pair antibody
Our Abpromise guarantee covers the use of ab64159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5.2F] to MyoD1 (ab16148). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [5.2F] to MyoD1 (ab16148) as Capture.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).|
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
- Information by UniProt
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
ab64159 staining MyoD1 in Mouse primary adult muscle stem cells (satellite cells) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 45 minutes at 21°C. Samples were incubated with primary antibody (1/250 in 5% Goat serum + 2% BSA + 0.2% Triton X-100) for 18 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
All lanes : Anti-MyoD1 antibody (ab64159) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate at 20 µg
Lane 2 : Rh30 Whole Cell Lysate at 5 µg
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa (possible post-translational modification)
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab64159 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab64159 detects a band at 45 kDa, while this differs to its predicted molecular weight of 34kDa, the banding pattern observed is consistent with what has been described in the literature PMID:19352326.
This product has been referenced in:
- Zhang P et al. Salidroside Inhibits Myogenesis by Modulating p-Smad3-Induced Myf5 Transcription. Front Pharmacol 9:209 (2018). Read more (PubMed: 29593538) »
- Mota R et al. Increasing Cardiomyocyte Atrogin-1 Reduces Aging-Associated Fibrosis and Regulates Remodeling in Vivo. Am J Pathol 188:1676-1692 (2018). Read more (PubMed: 29758183) »