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    myod1-antibody-epr6653-131-ab133627.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH
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RecombinantRabMAb

Recombinant Anti-MyoD1 antibody [EPR6653-131] (ab133627)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
  • Western blot - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
  • Anti-MyoD1 antibody [EPR6653-131] (ab133627)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6653-131] to MyoD1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-MyoD1 antibody [EPR6653-131]
    See all MyoD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6653-131] to MyoD1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human MyoD1 aa 50-150.
    Database link: P15172

  • Positive control

    • This antibody gave a positive signal in Human Rhabdomyosarcoma tissue as well as the Human Rhabdomyosarcoma Cell Line Rh30.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6653-131
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Muscle

Associated products

  • Alternative Versions

    • Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (ab240073)
  • Assay kits

    • MyoD Transcription Factor Assay Kit (Colorimetric) (ab207214)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human MyoD1 protein (ab114214)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).

Abcam recommends using milk as the blocking agent.

IHC-P (4)
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).

Abcam recommends using milk as the blocking agent.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
    Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P15172 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4654 Human
    • Omim: 159970 Human
    • SwissProt: P15172 Human
    • Unigene: 181768 Human
    • Alternative names

      • bHLHc1 antibody
      • Class C basic helix-loop-helix protein 1 antibody
      • MYF 3 antibody
      • Myf-3 antibody
      • MYF3 antibody
      • Myoblast determination protein 1 antibody
      • Myod 1 antibody
      • MYOD antibody
      • MYOD1 antibody
      • MYOD1_HUMAN antibody
      • Myogenic differentiation 1 antibody
      • Myogenic factor 3 antibody
      • Myogenic factor MYF 3 antibody
      • Myogenin D1 antibody
      • PUM antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)

      Immunohistochemical analysis of MyoD1 in paraffin embedded Human rhabdomyosarcoma tissue, using ab133627 at a dilution of 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
      Western blot - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
      All lanes : Anti-MyoD1 antibody [EPR6653-131] (ab133627) at 1/1000 dilution

      Lane 1 : Rh30 (Human Rhabdomyosarcoma) Whole Cell Lysate at 5 µg
      Lane 2 : Rh30 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?


      Exposure time: 4 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab133627 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

      ab133627 detects a band at 45 kDa, while this differs to its predicted molecular weight of 34 kDa, the banding pattern observed is consistent with what has been described in the literature PMID:19352326.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)

      ab133627 showing negative staining in Normal heart tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)

      ab133627 showing negative staining in Thyroid gland carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)

      ab133627 showing negative staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)

      ab133627 showing negative staining in Skeletal muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-MyoD1 antibody [EPR6653-131] (ab133627)
      Anti-MyoD1 antibody [EPR6653-131] (ab133627)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab133627? Please let us know so that we can cite the reference in this datasheet.

    ab133627 has been referenced in 4 publications.

    • Shelton M  et al. Gene expression profiling of skeletal myogenesis in human embryonic stem cells reveals a potential cascade of transcription factors regulating stages of myogenesis, including quiescent/activated satellite cell-like gene expression. PLoS One 14:e0222946 (2019). PubMed: 31560727
    • Tenente IM  et al. Myogenic regulatory transcription factors regulate growth in rhabdomyosarcoma. Elife 6:N/A (2017). PubMed: 28080960
    • Hoedt A  et al. Satellite cell response to erythropoietin treatment and endurance training in healthy young men. J Physiol 594:727-43 (2016). IHC ; Human . PubMed: 26607845
    • Farup J  et al. Pericyte response to contraction mode-specific resistance exercise training in human skeletal muscle. J Appl Physiol (1985) 119:1053-63 (2015). PubMed: 26404620

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-MyoD1 antibody [EPR6653-131]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skeletal muscle tissue)
    Permeabilization
    No
    Specification
    Skeletal muscle tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Reinnier Padilla

    Verified customer

    Submitted Sep 05 2018

    IHC - Wholemount abreview for Anti-MyoD1 antibody [EPR6653-131]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (intestine organoids)
    Specification
    intestine organoids
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 11 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyoD1 antibody [EPR6653-131]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 16 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyoD1 antibody [EPR6653-131]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% Normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 16 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyoD1 antibody [EPR6653-131]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (lungs)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    lungs
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 16 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-MyoD1 antibody [EPR6653-131]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Dako IHC kit as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Human Tissue sections (Mesenchymal stem cell)
    Specification
    Mesenchymal stem cell
    Permeabilization
    No
    Fixative
    liquid nitrogen
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 08 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyoD1 antibody [EPR6653-131]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (rhabdomyosarcoma)
    Specification
    rhabdomyosarcoma
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6.0 (Leica ER1) 20 min 100C
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 08 2013

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