Recombinant Anti-MyoD1 antibody [EPR6653-131] (ab133627)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147518-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6653-131] to MyoD1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-MyoD1 antibody [EPR6653-131]
See all MyoD1 primary antibodies -
Description
Rabbit monoclonal [EPR6653-131] to MyoD1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human MyoD1 aa 50-150.
Database link: P15172 -
Positive control
- This antibody gave a positive signal in Human Rhabdomyosarcoma tissue as well as the Human Rhabdomyosarcoma Cell Line Rh30.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6653-131 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
Abcam recommends using milk as the blocking agent. |
|
| IHC-P | (4) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). Abcam recommends using milk as the blocking agent. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4654 Human
- Omim: 159970 Human
- SwissProt: P15172 Human
- Unigene: 181768 Human
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Alternative names
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)
Immunohistochemical analysis of MyoD1 in paraffin embedded Human rhabdomyosarcoma tissue, using ab133627 at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-258260-anti-myod1-antibody-epr6653-131-western-blot.jpg)
Western blot - Anti-MyoD1 antibody [EPR6653-131] (ab133627)All lanes : Anti-MyoD1 antibody [EPR6653-131] (ab133627) at 1/1000 dilution
Lane 1 : Rh30 (Human Rhabdomyosarcoma) Whole Cell Lysate at 5 µg
Lane 2 : Rh30 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab133627 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab133627 detects a band at 45 kDa, while this differs to its predicted molecular weight of 34 kDa, the banding pattern observed is consistent with what has been described in the literature PMID:19352326.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147517-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)ab133627 showing negative staining in Normal heart tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147516-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)ab133627 showing negative staining in Thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147515-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)ab133627 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-147514-anti-myod1-antibody-epr6653-131-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (ab133627)ab133627 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-MyoD1 antibody [EPR6653-131] (ab133627)](https://www.abcam.com/ps/products/133/ab133627/Images/ab133627-7-benefits-of-recombinant-antibodies.png)
Anti-MyoD1 antibody [EPR6653-131] (ab133627)
Protocols
References (4)
ab133627 has been referenced in 4 publications.
- Shelton M et al. Gene expression profiling of skeletal myogenesis in human embryonic stem cells reveals a potential cascade of transcription factors regulating stages of myogenesis, including quiescent/activated satellite cell-like gene expression. PLoS One 14:e0222946 (2019). PubMed: 31560727
- Tenente IM et al. Myogenic regulatory transcription factors regulate growth in rhabdomyosarcoma. Elife 6:N/A (2017). PubMed: 28080960
- Hoedt A et al. Satellite cell response to erythropoietin treatment and endurance training in healthy young men. J Physiol 594:727-43 (2016). IHC ; Human . PubMed: 26607845
- Farup J et al. Pericyte response to contraction mode-specific resistance exercise training in human skeletal muscle. J Appl Physiol (1985) 119:1053-63 (2015). PubMed: 26404620
