Recombinant Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (ab240073)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6653-131] to MyoD1 - BSA and Azide free
- Suitable for: WB, ChIC/CUT&RUN-seq, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free
See all MyoD1 primary antibodies -
Description
Rabbit monoclonal [EPR6653-131] to MyoD1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ChIC/CUT&RUN-seq, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human Rhabdomyosarcoma tissue WB: Human Rhabdomyosarcoma Cell Line Rh30, RD (Human muscle rhabdomyosarcoma) whole cell lysate ICC/IF: RD (Human muscle rhabdomyosarcoma) cell line. ChIC/CUT&RUN-Seq: RD (Human muscle rhabdomyosarcoma) cells.
-
General notes
ab240073 is the carrier-free version of ab133627.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6653-131 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-MyoD1 antibody [EPR6653-131] (ab133627)
- Alexa Fluor® 488 Anti-MyoD1 antibody [EPR6653-131] (ab311001)
- Alexa Fluor® 647 Anti-MyoD1 antibody [EPR6653-131] (ab311127)
- Alexa Fluor® 594 Anti-MyoD1 antibody [EPR6653-131] (ab311759)
- Alexa Fluor® 568 Anti-MyoD1 antibody [EPR6653-131] (ab313039)
- Alexa Fluor® 555 Anti-MyoD1 antibody [EPR6653-131] (ab313240)
-
Assay kits
-
Conjugation kits
-
Immunohistochemistry kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
Abcam recommends using milk as the blocking agent. |
|
ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). Abcam recommends using milk as the blocking agent. |
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4654 Human
- Omim: 159970 Human
- SwissProt: P15172 Human
- Unigene: 181768 Human
-
Alternative names
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
Images
-
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 RD (Human muscle rhabdomyosarcoma) cells and 5µg of ab133627 [EPR6653-131]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).
-
Immunohistochemical analysis of MyoD1 in paraffin embedded Human rhabdomyosarcoma tissue, using ab133627 at a dilution of 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133627 showing negative staining in Normal heart tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133627 showing negative staining in Thyroid gland carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133627 showing negative staining in Normal kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133627 showing negative staining in Skeletal muscle tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab240073 has not yet been referenced specifically in any publications.