• Product name

    Anti-Myoferlin antibody [7D2]
    See all Myoferlin primary antibodies
  • Description

    Mouse monoclonal [7D2] to Myoferlin
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Myoferlin (N terminal).
    Database link: Q9NZM1


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

  • Clone number

  • Isotype

  • Light chain type

  • Research areas


Our Abpromise guarantee covers the use of ab76746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100 - 1/1000.
Flow Cyt 1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


WB 1/500 - 1/5000. Predicted molecular weight: 235 kDa.


  • Function

    Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR.
  • Tissue specificity

    Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.
  • Sequence similarities

    Belongs to the ferlin family.
    Contains 5 C2 domains.
  • Domain

    The C2 domain 1 associates with lipid membranes in a calcium-dependent manner.
  • Cellular localization

    Cell membrane. Nucleus membrane. Cytoplasmic vesicle membrane. Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fer 1 like 3, myoferlin (C. elegans) antibody
    • Fer 1 like family member 3 antibody
    • Fer 1 like protein 3 antibody
    • Fer-1-like protein 3 antibody
    • FER1L3 antibody
    • myoF antibody
    • MYOF_HUMAN antibody
    • Myoferlin antibody
    see all


  • Immunohistochemical analysis of Human airway tissue, staining Myoferlin with ab76746 at 1/500 dilution. Non-specific binding was blocked with 10% horse serum and slides were incubated overnight with primary antibody. Staining was detected with DAB.
  • ICC/IF image of ab76746 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab76746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76746, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:

  • Song DH  et al. Prognostic role of myoferlin expression in patients with clear cell renal cell carcinoma. Oncotarget 8:89033-89039 (2017). IHC-P ; Human . Read more (PubMed: 29179496) »
  • Piper AK  et al. Enzymatic cleavage of myoferlin releases a dual C2-domain module linked to ERK signalling. Cell Signal 33:30-40 (2017). Read more (PubMed: 28192161) »
See all 5 Publications for this product

Customer reviews and Q&As

Flow Cytometry
Mouse Cell (B16 melanoma tumour cell line)
Gating Strategy
live cells, singlets
B16 melanoma tumour cell line
Cell harvesting/tissue preparation method: cells were trypsinised, washed with PBS
Sample buffer: PBS

Abcam user community

Verified customer

Submitted Sep 12 2017

For licensing inquiries, please contact partnerships@abcam.com

Sign up