Overview

  • Product name

  • Description

    Rabbit polyclonal to Myoferlin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Myoferlin aa 700-750. The exact sequence is proprietary. NP_038479.1
    Sequence:

    E DTRYTLPLTE GKANVTVLDT QIRKLRSRSL SQIHEAAVRM RSEATDVKST


    Database link: Q9NZM1

  • Positive control

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/5000. Predicted molecular weight: 235 kDa.

Target

  • Function

    Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR.
  • Tissue specificity

    Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.
  • Sequence similarities

    Belongs to the ferlin family.
    Contains 5 C2 domains.
  • Domain

    The C2 domain 1 associates with lipid membranes in a calcium-dependent manner.
  • Cellular localization

    Cell membrane. Nucleus membrane. Cytoplasmic vesicle membrane. Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fer 1 like 3, myoferlin (C. elegans) antibody
    • Fer 1 like family member 3 antibody
    • Fer 1 like protein 3 antibody
    • Fer-1-like protein 3 antibody
    • FER1L3 antibody
    • myoF antibody
    • MYOF_HUMAN antibody
    • Myoferlin antibody
    see all

Images

  • Anti-Myoferlin antibody (ab190264) at 0.4 µg/ml + Hela whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 235 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation of Hela whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling Myoferlin with ab190264 at 1 µg/ml by western blot.  Myoferlin was immunoprecipitated using a control antibody (1) or ab190264 (2) at 6 µg per reaction.  Lane 3 shows IgG control.  

References

ab190264 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle (combined muscle from mouse hind l)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE gel)
Loading amount
25 µg
Specification
Skeletal muscle (combined muscle from mouse hind l
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 30 2017

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