Key features and details
- Rabbit polyclonal to Myoferlin
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Myoferlin antibody
See all Myoferlin primary antibodies
DescriptionRabbit polyclonal to Myoferlin
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Chimpanzee, Gorilla, Orangutan
- HeLa whole cell lysate (ab150035)
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 235 kDa.|
FunctionCalcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR.
Tissue specificityExpressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.
Sequence similaritiesBelongs to the ferlin family.
Contains 5 C2 domains.
DomainThe C2 domain 1 associates with lipid membranes in a calcium-dependent manner.
Cellular localizationCell membrane. Nucleus membrane. Cytoplasmic vesicle membrane. Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures.
- Information by UniProt
- Fer 1 like 3, myoferlin (C. elegans) antibody
- Fer 1 like family member 3 antibody
- Fer 1 like protein 3 antibody
Anti-Myoferlin antibody (ab190264) at 0.4 µg/ml + Hela whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 235 kDa
Exposure time: 3 minutes
Immunoprecipitation of Hela whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling Myoferlin with ab190264 at 1 µg/ml by western blot. Myoferlin was immunoprecipitated using a control antibody (1) or ab190264 (2) at 6 µg per reaction. Lane 3 shows IgG control.
ab190264 has not yet been referenced specifically in any publications.