Anti-Myogenin antibody (ab103924)

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Overview

  • Product name

    Anti-Myogenin antibody
    See all Myogenin primary antibodies

  • Description

    Goat polyclonal to Myogenin

  • Host species

    Goat

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Cow, Dog, Human, Pig

  • Immunogen

    Synthetic peptide:

    C-TDAHNLHSLTSIVD

    , corresponding to C terminal amino acids 193-206 of Human Myogenin (NP_002470.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human Myogenin protein (ab114244) can be used as a positive control in WB. Mouse Skeletal Muscle lysate

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab103924 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 25 kDa).

Target

Images

  • Western blot - Anti-Myogenin antibody (ab103924)
    Western blot - Anti-Myogenin antibody (ab103924)
    Anti-Myogenin antibody (ab103924) at 2 µg/ml + Mouse Skeletal Muscle lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 25 kDa
    Observed band size: ~38 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Zhang W  et al. Transcription factor EGR1 promotes differentiation of bovine skeletal muscle satellite cells by regulating MyoG gene expression. J Cell Physiol 233:350-362 (2018). Read more (PubMed: 28256014) »
See 1 Publication for this product

Publishing research using ab103924? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Myogenin antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (muscle precursor cells/myotubes)
Permeabilization
No
Specification
muscle precursor cells/myotubes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Methanol
Read More

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Verified customer

Submitted Aug 18 2016

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