- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Myogenin antibody
See all Myogenin primary antibodies -
DescriptionGoat polyclonal to Myogenin
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Host speciesGoat
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Rat, Sheep, Rabbit, Goat, Cow, Dog, Human, Pig
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Immunogen
Synthetic peptide:
C-TDAHNLHSLTSIVD
, corresponding to C terminal amino acids 193-206 of Human Myogenin (NP_002470.2). -
Positive control
- Mouse Skeletal Muscle lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab103924 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab103924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 25 kDa). |
Target
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FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
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Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 281343 Cow
- Entrez Gene: 490224 Dog
- Entrez Gene: 4656 Human
- Entrez Gene: 17928 Mouse
- Entrez Gene: 497618 Pig
- Entrez Gene: 29148 Rat
- Entrez Gene: 443158 Sheep
- Omim: 159980 Human
see all -
Alternative names
- bHLHc3 antibody
- cb553 antibody
- Class C basic helix-loop-helix protein 3 antibody
see all
Images
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Western blot - Anti-Myogenin antibody (ab103924)Anti-Myogenin antibody (ab103924) at 2 µg/ml + Mouse Skeletal Muscle lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: ~38 kDa (why is the actual band size different from the predicted?)
Protocols
References
ab103924 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"