Anti-Myogenin antibody (ab103924)
Key features and details
- Goat polyclonal to Myogenin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Myogenin antibody
See all Myogenin primary antibodies -
Description
Goat polyclonal to Myogenin -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Sheep, Rabbit, Goat, Cow, Dog, Human, Pig
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Immunogen
Synthetic peptide:
C-TDAHNLHSLTSIVD
, corresponding to C terminal amino acids 193-206 of Human Myogenin (NP_002470.2). -
Positive control
- Recombinant Human Myogenin protein (ab114244) can be used as a positive control in WB. Mouse Skeletal Muscle lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab103924 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab103924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 25 kDa). A 1 hour primary incubation is recommended for this product. |
Target
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Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281343 Cow
- Entrez Gene: 490224 Dog
- Entrez Gene: 4656 Human
- Entrez Gene: 17928 Mouse
- Entrez Gene: 497618 Pig
- Entrez Gene: 29148 Rat
- Entrez Gene: 443158 Sheep
- Omim: 159980 Human
see all -
Alternative names
- bHLHc3 antibody
- cb553 antibody
- Class C basic helix-loop-helix protein 3 antibody
see all
Images
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Western blot - Anti-Myogenin antibody (ab103924)Anti-Myogenin antibody (ab103924) at 2 µg/ml + Mouse Skeletal Muscle lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: ~38 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (1)
ab103924 has been referenced in 1 publication.
- Zhang W et al. Transcription factor EGR1 promotes differentiation of bovine skeletal muscle satellite cells by regulating MyoG gene expression. J Cell Physiol 233:350-362 (2018). PubMed: 28256014
