The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
This antibody has low affinity on skeletal muscle samples in WB. Please increase protein or antibody amount to get satisfied results.
WB, IHC-Pmore details Unsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
Synthetic peptide within Human Myogenin. The exact sequence is proprietary.
WB: C2C12 and Human fetal muscle lysates;
IHC-P: Human rhabdomyosarcoma tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human rhabdomyosarcoma tissue sections labeling Myogenin with Purified ab124800 at 1:500 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-Myogenin antibody [EPR4789] (ab124800)
All lanes : Anti-Myogenin antibody [EPR4789] (ab124800) at 0.5 µg/ml (purified)
Lane 1 : Human fetal skeletal muscle lysates Lane 2 : Rat fetal skeletal muscle lysates Lane 3 : Mouse fetal skeletal muscle lysates Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates Lane 5 : C2C12 (Mouse myoblasts myoblast) treated with 2% horse serum whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 25 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
This antibody has low affinity on skeletal muscle samples. Please increase protein or antibody amount to get satisfied results. Myogenin expression is upregulated in C2C12 cells during differentiation process induced by horse serum treatment (PMID: 26563778).
Akiyama T et al. Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing. Sci Rep8:1189 (2018).
Read more (PubMed: 29352121) »