Recombinant
RabMAb

Anti-Myogenin antibody [EPR4789] (ab124800)

Overview

  • Product name
    Anti-Myogenin antibody [EPR4789]
    See all Myogenin primary antibodies
  • Description
    Rabbit monoclonal [EPR4789] to Myogenin
  • Host species
    Rabbit
  • Specificity
    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. This antibody has low affinity on skeletal muscle samples in WB. Please increase protein or antibody amount to get satisfied results.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Myogenin. The exact sequence is proprietary.

  • Positive control
    • WB: C2C12 and Human fetal muscle lysates; IHC-P: Human rhabdomyosarcoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa).

For unpurified use at 1/1000.00000 - 1/10000.00000

This antibody has low affinity on skeletal muscle samples in WB. Please increase protein or antibody amount to get satisfied results.

IHC-P 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100.00000 - 1/250.00000

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human rhabdomyosarcoma tissue sections labeling Myogenin with Purified ab124800 at 1:500 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
    • All lanes : Anti-Myogenin antibody [EPR4789] (ab124800) at 0.5 µg/ml (purified)

      Lane 1 : Human fetal skeletal muscle lysates
      Lane 2 : Rat fetal skeletal muscle lysates
      Lane 3 : Mouse fetal skeletal muscle lysates
      Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
      Lane 5 : C2C12 (Mouse myoblasts myoblast) treated with 2% horse serum whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 25 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

      This antibody has low affinity on skeletal muscle samples. Please increase protein or antibody amount to get satisfied results.
      Myogenin expression is upregulated in C2C12 cells during differentiation process induced by horse serum treatment (PMID: 26563778).

    • Unpurified ab124800, at a 1/100 dilution, staining Myogenin in paraffin embedded Human rhabdomyosarcoma tissue by Immunohistochemistry.

    References

    This product has been referenced in:
    • Akiyama T  et al. Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing. Sci Rep 8:1189 (2018). Read more (PubMed: 29352121) »
    • Zhang P  et al. Salidroside Inhibits Myogenesis by Modulating p-Smad3-Induced Myf5 Transcription. Front Pharmacol 9:209 (2018). Read more (PubMed: 29593538) »

    See all 12 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse embryonic stem cells differentiated for 30 d)
    Permeabilization
    Yes - 0.5% Triton
    Specification
    mouse embryonic stem cells differentiated for 30 d
    Blocking step
    PBS+0.1%Triton+ 0.1%BSA + 10% Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 16 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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