Recombinant Anti-Myogenin antibody [EPR4789] (ab124800)
![Immunocytochemistry - Anti-Myogenin antibody [EPR4789] (ab124800)](https://www.abcam.com/ps/products/124/ab124800/Images/ab124800-7-anti-myogenin-immunocytochemistry-ips-myocytes-human-antibody-epr4789.jpg "Immunocytochemistry - Anti-Myogenin antibody [EPR4789] (ab124800)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4789] to Myogenin
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Myogenin antibody [EPR4789]
See all Myogenin primary antibodies -
Description
Rabbit monoclonal [EPR4789] to Myogenin -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. This antibody has low affinity on skeletal muscle samples in WB. Please increase protein or antibody amount to get satisfied results. -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Myogenin. The exact sequence is proprietary.
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Positive control
- WB: C2C12 and Human fetal muscle lysates; IHC-P: Human rhabdomyosarcoma tissue; ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4789 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 0.5 - 5 µg/ml.
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| WB |
1/200. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa).
For unpurified use at 1/1000.00000 - 1/10000.00000 This antibody has low affinity on skeletal muscle samples in WB. Please increase protein or antibody amount to get satisfied results. |
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| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100.00000 - 1/250.00000 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
| Notes |
|---|
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ICC
Use a concentration of 0.5 - 5 µg/ml. |
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WB
1/200. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa). For unpurified use at 1/1000.00000 - 1/10000.00000 This antibody has low affinity on skeletal muscle samples in WB. Please increase protein or antibody amount to get satisfied results. |
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IHC-P
1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100.00000 - 1/250.00000 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4656 Human
- Entrez Gene: 17928 Mouse
- Entrez Gene: 29148 Rat
- Omim: 159980 Human
- SwissProt: P15173 Human
- SwissProt: P12979 Mouse
- SwissProt: P20428 Rat
- Unigene: 2830 Human
see all -
Alternative names
- bHLHc3 antibody
- cb553 antibody
- Class C basic helix-loop-helix protein 3 antibody
see all
Images
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Immunocytochemistry - Anti-Myogenin antibody [EPR4789] (ab124800)
Immunofluorescence staining of Myogenin using ab124800 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 3 days post induction.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab124800 at 0.5 µg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab124800 gave comparable results using MeOH fixation (100%, 5 min).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)](https://www.abcam.com/ps/products/124/ab124800/Images/ab124800-314297-anti-myogenin-antibody-epr4789-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human rhabdomyosarcoma tissue sections labeling Myogenin with Purified ab124800 at 1:500 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain -
![Western blot - Anti-Myogenin antibody [EPR4789] (ab124800)](https://www.abcam.com/ps/products/124/ab124800/Images/ab124800-314537-anti-myogenin-antibody-epr4789-western-blot.jpg)
Western blot - Anti-Myogenin antibody [EPR4789] (ab124800)All lanes : Anti-Myogenin antibody [EPR4789] (ab124800) at 0.5 µg/ml (purified)
Lane 1 : Human fetal skeletal muscle lysates
Lane 2 : Rat fetal skeletal muscle lysates
Lane 3 : Mouse fetal skeletal muscle lysates
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 5 : C2C12 (Mouse myoblasts myoblast) treated with 2% horse serum whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 25 kDaBlocking and diluting buffer: 5% NFDM/TBST.
This antibody has low affinity on skeletal muscle samples. Please increase protein or antibody amount to get satisfied results.
Myogenin expression is upregulated in C2C12 cells during differentiation process induced by horse serum treatment (PMID: 26563778). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)](https://www.abcam.com/ps/products/124/ab124800/Images/ab124800-138695-anti-myogenin-antibody-epr4789-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)Unpurified ab124800, at a 1/100 dilution, staining Myogenin in paraffin embedded Human rhabdomyosarcoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Anti-Myogenin antibody [EPR4789] (ab124800)](https://www.abcam.com/ps/products/124/ab124800/Images/ab124800-6-benefits-of-recombinant-antibodies.png)
Anti-Myogenin antibody [EPR4789] (ab124800)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab124800 has been referenced in 33 publications.
- Elnour IE et al. Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400. Cells 10:N/A (2021). PubMed: 33467116
- Norizadeh Abbariki T et al. The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation. Sci Rep 11:12904 (2021). PubMed: 34145356
- He P et al. The changing mouse embryo transcriptome at whole tissue and single-cell resolution. Nature 583:760-767 (2020). PubMed: 32728245
- Chung LH et al. High phosphate induces skeletal muscle atrophy and suppresses myogenic differentiation by increasing oxidative stress and activating Nrf2 signaling. Aging (Albany NY) 12:21446-21468 (2020). PubMed: 33136552
- Paris ND et al. Chemoradiation impairs myofiber hypertrophic growth in a pediatric tumor model. Sci Rep 10:19501 (2020). PubMed: 33177579