- Datasheet
- Specific References (32)
- Protocols
Overview
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Product nameAnti-Myogenin antibody [F5D]
See all Myogenin primary antibodies -
DescriptionMouse monoclonal [F5D] to Myogenin
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Host speciesMouse
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, WB, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Rat, Human, Pig
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Immunogen
BALB/ C mice were injected with recombinant protein containing rat myogenin amino acid 30 - 224.
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Positive control
- Rhabdomyosarcoma
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityMonoclonal
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Clone numberF5D
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab1835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50. | |
| ICC/IF | 1/50. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommended starting point for ab1835 incubation is 30min RT. | |
| WB | 1/250. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa). Abcam recommends using BSA as the blocking agent. |
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| IHC-FoFr | Use a concentration of 1 µg/ml. |
Target
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FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
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Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 4656 Human
- Entrez Gene: 17928 Mouse
- Entrez Gene: 497618 Pig
- Entrez Gene: 29148 Rat
- Omim: 159980 Human
- SwissProt: P15173 Human
- SwissProt: P12979 Mouse
- SwissProt: P49812 Pig
see all -
Alternative names
- bHLHc3 antibody
- cb553 antibody
- Class C basic helix-loop-helix protein 3 antibody
see all
Images
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![Western blot - Anti-Myogenin antibody [F5D] (ab1835)](https://www.abcam.com/ps/products/1/ab1835/Images/ab1835-243328-WBGR1874861.jpg)
Western blot - Anti-Myogenin antibody [F5D] (ab1835)All lanes : Anti-Myogenin antibody [F5D] (ab1835) at 1/250 dilution
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 40 kDa (why is the actual band size different from the predicted?)
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1835 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myogenin antibody [F5D] (ab1835)](https://www.abcam.com/ps/products/1/ab1835/Images/Myogenin-Primary-antibodies-ab1835-5.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myogenin antibody [F5D] (ab1835)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomAdult mouse muscle section stained with ab1835. The animals were perfused with 4% PFA. The sections were incubated in 5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab1835, 1µg/ml) overnight at +4°C. The secondary antibody Alexa Fluor® 568 donkey anti-mouse IgG (H+L) (red) was used at a 1/1000 dilution for 1h. -
![Immunocytochemistry/ Immunofluorescence - Anti-Myogenin antibody [F5D] (ab1835)](https://www.abcam.com/ps/products/1/ab1835/Images/ab1835_3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Myogenin antibody [F5D] (ab1835)This image is courtesy of an Abreview submitted by Dr Mal Niladriab1835 at 1/50 dilution staining pig differentiated skeletal myoblast cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized with Triton-X100 and incubated with the antibody overnight at 4C. An Alexa Fluor 488 conjugated goat anti-mouse antibody was used as the secondary. The image was captured using a confocal laser scanning microscope with an additional Differential Interference Contrast (DIC) mode. The image shows DAPI nuclear counterstain (blue, upper left panel), Myogenin staining (green, upper right panel), DIC (phase) image (lower left panel) and overlay (lower right panel).
Myogenin is not expressed in proliferating myoblasts, but rather in mononucleated differentiating myoblasts (See abreview for more detail).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [F5D] (ab1835)](https://www.abcam.com/ps/products/1/ab1835/Images/ab1835_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [F5D] (ab1835)ab1835 - immunohistochemistry
Formalin fixed paraffin embedded human rhabdomyosarcoma stained with Myogenin using ABC and AEC chromogen.
Protocols
References
This product has been referenced in:
- Miersch C et al. Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation. BMC Cell Biol 19:2 (2018). Flow Cyt . Read more (PubMed: 29523096) »
- Park S et al. Autophagy induction in the skeletal myogenic differentiation of human tonsil-derived mesenchymal stem cells. Int J Mol Med 39:831-840 (2017). Read more (PubMed: 28259927) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"