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    myogenin-antibody-f5d-ab1835.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH
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Anti-Myogenin antibody [F5D] (ab1835)

  • Datasheet
  • SDS
Reviews (10)Q&A (3)References (63)

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Immunocytochemistry/ Immunofluorescence - Anti-Myogenin antibody [F5D] (ab1835)
  • Western blot - Anti-Myogenin antibody [F5D] (ab1835)
  • Immunocytochemistry - Anti-Myogenin antibody [F5D] (ab1835)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [F5D] (ab1835)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myogenin antibody [F5D] (ab1835)

Key features and details

  • Mouse monoclonal [F5D] to Myogenin
  • Suitable for: IHC-FoFr, ICC, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human Myogenin protein (ab114244)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Cells
Product image
ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)

View more associated products

Overview

  • Product name

    Anti-Myogenin antibody [F5D]
    See all Myogenin primary antibodies
  • Description

    Mouse monoclonal [F5D] to Myogenin
  • Host species

    Mouse
  • Specificity

    The immunogen used to raise this antibody has 100% homology with the Rat Myogenin protein. Some customers have successfully used ab1835 on rat samples, however we have not been successful detecting Myogenin in this species in our own testing and therefore cannot guarantee rat reactivity. Please contact Abcam Scientific Support for more information.

  • Tested applications

    Suitable for: IHC-FoFr, ICC, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Recombinant full length protein corresponding to Rat Myogenin aa 1-250.
    Database link: P20428

  • Positive control

    • IHC-P: Human Rhabdomyosarcoma; ICC: 2 days differentiated C2C12 cells, ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612);
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    F5D
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis

Associated products

  • Alternative Versions

    • Anti-Myogenin antibody [F5D] - BSA and Azide free (ab212667)
    • Anti-Myogenin antibody [F5D] - BSA and Azide free (ab264517)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Myogenin protein (ab114244)
  • Related Products

    • ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr (1)
Use at an assay dependent concentration.
ICC
Use a concentration of 1 - 5 µg/ml.
IHC-P (1)
Use at an assay dependent concentration.
WB (3)
1/250 - 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).
Notes
IHC-FoFr
Use at an assay dependent concentration.
ICC
Use a concentration of 1 - 5 µg/ml.
IHC-P
Use at an assay dependent concentration.
WB
1/250 - 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).

Target

  • Function

    Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P15173 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4656 Human
    • Entrez Gene: 17928 Mouse
    • Entrez Gene: 497618 Pig
    • Entrez Gene: 29148 Rat
    • Omim: 159980 Human
    • SwissProt: P15173 Human
    • SwissProt: P12979 Mouse
    • SwissProt: P49812 Pig
    • SwissProt: P20428 Rat
    • Unigene: 2830 Human
    • Unigene: 16528 Mouse
    • Unigene: 9465 Rat
    see all
  • Alternative names

    • bHLHc3 antibody
    • cb553 antibody
    • Class C basic helix-loop-helix protein 3 antibody
    • MYF 4 antibody
    • Myf-4 antibody
    • MYF4 antibody
    • MYOG antibody
    • MYOG_HUMAN antibody
    • Myogenic factor 4 antibody
    • Myogenic factor 4 myogenin antibody
    • Myogenin antibody
    • Myogenin myogenin factor 4 antibody
    • OTTHUMP00000039094 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Myogenin antibody [F5D] (ab1835)
    Immunocytochemistry/ Immunofluorescence - Anti-Myogenin antibody [F5D] (ab1835)

    ab1835 staining Myogenin in undifferentiated C2C12 cells (top panel) and 2 days differentiated C2C12 cells (bottom panel).

    The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab1835 at 1 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution overnight at 4ºC. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Myogenin antibody [F5D] (ab1835)
    Western blot - Anti-Myogenin antibody [F5D] (ab1835)
    All lanes :

    Lane 1 : Differentiated C2C12 whole cell lysate - Day 0 control
    Lane 2 : Differentiated C2C12 whole cell lysate - Day 1
    Lane 3 : Differentiated C2C12 whole cell lysate - Day 2
    Lane 4 : Differentiated C2C12 whole cell lysate - Day 3
    Lane 5 : Differentiated C2C12 whole cell lysate - Day 4
    Lane 6 : Differentiated C2C12 whole cell lysate - Day 5
    Lane 7 : Differentiated C2C12 whole cell lysate - Day 6

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?



    C2C12 cells were differentiated into myotubes as previously described in PMID:26563778.

    Lanes 1-7: Merged signal (red and green). Green - ab1835 observed at 30kDa. Red - loading control ab181602 observed at 37kDa.

    This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab1835 and ab181602 (Rabbit anti GAPDH loading control), were incubated overnight at 4°C at a 1 in 400 dilution and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-Myogenin antibody [F5D] (ab1835)
    Immunocytochemistry - Anti-Myogenin antibody [F5D] (ab1835)

    Immunofluorescence staining of Myogenin using ab1835 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 3 days post induction.


    The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab1835 at 1 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.


    The antibody ab1835 gave comparable results using MeOH fixation (100%, 5 min).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [F5D] (ab1835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [F5D] (ab1835)

    IHC image of Myogenin staining in a section of formalin-fixed paraffin-embedded normal Human Rhabdomyosarcoma* performed on a Leica BOND™ system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab1835, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myogenin antibody [F5D] (ab1835)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myogenin antibody [F5D] (ab1835)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    Adult mouse muscle section stained with ab1835. The animals were perfused with 4% PFA. The sections were incubated in 5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab1835, 1µg/ml) overnight at +4°C. The secondary antibody Alexa Fluor® 568 donkey anti-mouse IgG (H+L) (red) was used at a 1/1000 dilution for 1h.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (63)

Publishing research using ab1835? Please let us know so that we can cite the reference in this datasheet.

ab1835 has been referenced in 63 publications.

  • Moroki T  et al. Databases for technical aspects of immunohistochemistry: 2021 update. J Toxicol Pathol 34:161-180 (2021). PubMed: 33976473
  • Bouredji Z  et al. Testing the efficacy of a human full-length OPG-Fc analog in a severe model of cardiotoxin-induced skeletal muscle injury and repair. Mol Ther Methods Clin Dev 21:559-573 (2021). PubMed: 33997104
  • Qi R  et al. The intestinal microbiota contributes to the growth and physiological state of muscle tissue in piglets. Sci Rep 11:11237 (2021). PubMed: 34045661
  • Perez-Puyana V  et al. Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning. Polymers (Basel) 13:N/A (2021). PubMed: 34066640
  • Olson LC  et al. Advanced Glycation End Products Are Retained in Decellularized Muscle Matrix Derived from Aged Skeletal Muscle. Int J Mol Sci 22:N/A (2021). PubMed: 34445538
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Myogenin antibody [F5D]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
30 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 05 2011

Western blot abreview for Anti-Myogenin antibody [F5D]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle)
Loading amount
20 µg
Specification
Muscle
Gel Running Conditions
Reduced Denaturing (12% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 24 2010

Western blot abreview for Anti-Myogenin antibody [F5D]

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (skeletal myotubes)
Loading amount
5 µg
Specification
skeletal myotubes
Gel Running Conditions
Reduced Denaturing (10 % gel, semi-dry)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Henrike Sell

Verified customer

Submitted Feb 02 2010

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