Overview

  • Product name

    Anti-Myogenin antibody [MGN185 + F5D]
    See all Myogenin primary antibodies
  • Description

    Mouse monoclonal [MGN185 + F5D] to Myogenin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cat, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Other Immunogen Type corresponding to Myogenin. Human myogenin recombinant protein (P15173) (Clone: MGN185); Rat Myogenin recombinant fragment containing amino acid 30-224 (Protein length = 287) (P20428) (Clone: F5D).

  • Positive control

    • Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

Properties

Applications

Our Abpromise guarantee covers the use of ab199017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

30 minutes at RT.

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

30 minutes at RT.

Flow Cyt Use 0.5-1µl for 106 cells.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IP Use at 1-2 µg/mg of lysate.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rhabdomyosarcoma labeling Myogenin with ab199017 at 1 µg/mL.

References

ab199017 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199017.
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