Overview

  • Product name

    Anti-Myogenin antibody [SPM144] - BSA and Azide free
    See all Myogenin primary antibodies
  • Description

    Mouse monoclonal [SPM144] to Myogenin - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IP, IHC-P, ICC/IF, Flow Cyt, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cat, Human, Pig
  • Immunogen

    Recombinant full length protein corresponding to Human Myogenin.
    Database link: P15173

  • Positive control

    • Rh-30 cells; skeletal muscle or rhabdomyosarcoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab212668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 1-2 µg/mg of lysate.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

for 30 minutes at room temperature.

ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use a concentration of 0.5 - 1 µg/ml.

Final volume 100 µL.

WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa).
ELISA Use at an assay dependent concentration.

(For coating)

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human rhabdomyosarcoma tissue section labeling myogenin with ab212668 at 1 µg/mL.

References

ab212668 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab212668.
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