Recombinant
RabMAb

Recombinant Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

Overview

  • Product name

    Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free
    See all Myoglobin primary antibodies
  • Description

    Rabbit monoclonal [EP3081Y] to Myoglobin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Myoglobin aa 100-200 (C terminal). The exact sequence is proprietary.
    Database link: P02144

  • Positive control

    • IHC-P: Mouse cardiac muscle tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab232388 is a PBS-only buffer format of ab77232. Please refer to ab77232 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • Purified ab77232 staining Myoglobin in Human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Purified ab77232 staining Myoglobin in Rat cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Purified ab77232 staining Myoglobin in the C2C12 (mouse myoblasts myoblast) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (ab150077) was used as the secondary antibody. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) and DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Unpurified ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Unpurified ab77232 showing positive staining in Skeletal muscle tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Unpurified ab77232 showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Unpurified ab77232 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Unpurified ab77232 showing negative staining in Normal liver tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Produced using unpurified ab77232.

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Purified ab77232 staining Myoglobin in mouse cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A ready to use HRP-conjugated Goat Anti-Rabbit IgG H&L was used as the secondary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

References

ab232388 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232388.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up