Product nameAnti-Myoglobin antibody [EP3081Y] (HRP)
See all Myoglobin primary antibodies
DescriptionRabbit monoclonal [EP3081Y] to Myoglobin (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Myoglobin (C terminal). The exact sequence is proprietary.
- WB: Human, rat and mouse heart muscle tissue lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Dissociation constant (KD)KD = 1.78 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab199243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
FunctionServes as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Sequence similaritiesBelongs to the globin family.
- Information by UniProt
- MB antibody
- MGC13548 antibody
- MYG_HUMAN antibody
- Myoglobin antibody
All lanes : Anti-Myoglobin antibody [EP3081Y] (HRP) (ab199243) at 1/5000 dilution
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 2 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199243 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab199243 has not yet been referenced specifically in any publications.