Recombinant
RabMAb

Recombinant Anti-Myoglobin antibody [EP3081Y] (HRP) (ab199243)

Overview

  • Product name

    Anti-Myoglobin antibody [EP3081Y] (HRP)
    See all Myoglobin primary antibodies
  • Description

    Rabbit monoclonal [EP3081Y] to Myoglobin (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Myoglobin (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human, rat and mouse heart muscle tissue lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab199243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Target

Images

  • All lanes : Anti-Myoglobin antibody [EP3081Y] (HRP) (ab199243) at 1/5000 dilution

    Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa


    Exposure time: 2 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199243 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab199243 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199243.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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