Overview

  • Product name

    Anti-MYOM1 antibody [EPR17322-9]
    See all MYOM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR17322-9] to MYOM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human MYOM1 aa 1450 to the C-terminus. The exact sequence is proprietary.
    Database link: P52179

  • Positive control

    • WB; Human fetal heart lysate; mouse heart and muscle lysates; rat heart lysate. IHC-P; Human cardiac muscle, mouse cardiac muscle and rat skeletal muscle tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 188 kDa (predicted molecular weight: 188 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.
  • Sequence similarities

    Contains 5 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • 190 kDa connectin-associated protein antibody
    • 190 kDa titin-associated protein antibody
    • EH myomesin antibody
    • MGC134946 antibody
    • MGC134947 antibody
    • MYOM1 antibody
    • MYOM1_HUMAN antibody
    • Myomesin (M protein) 1 (190kD) antibody
    • Myomesin 1 (skelemin) 185kDa antibody
    • Myomesin 1 185kDa antibody
    • Myomesin 1, 185kDa antibody
    • Myomesin family member 1 antibody
    • Myomesin-1 antibody
    • Skelemin antibody
    see all

Images

  • Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution + Human fetal heart lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 188 kDa
    Observed band size: 188 kDa


    Exposure time: 2 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 188 kDa
    Observed band size: 188 kDa


    Exposure time: 5 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution

    Lane 1 : Rat heart lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

    Predicted band size: 188 kDa
    Observed band size: 188 kDa


    Exposure time: 5 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human cardiac muscle tissue is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on human tonsil tissue is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

See 1 Publication for this product

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