RecombinantRabMAb

Recombinant Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)

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Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
  • Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
  • Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
  • Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17322-9] to MYOM1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free
    See all MYOM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17322-9] to MYOM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human MYOM1 aa 1450 to the C-terminus. The exact sequence is proprietary.
    Database link: P52179

  • General notes

    Ab251439 is the carrier-free version of ab205618. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251439 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab251439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 188 kDa (predicted molecular weight: 188 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.

  • Sequence similarities

    Contains 5 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Information by UniProt

  • Database links

    • Alternative names

      • 190 kDa connectin-associated protein antibody
      • 190 kDa titin-associated protein antibody
      • EH myomesin antibody
      • MGC134946 antibody
      • MGC134947 antibody
      • MYOM1 antibody
      • MYOM1_HUMAN antibody
      • Myomesin (M protein) 1 (190kD) antibody
      • Myomesin 1 (skelemin) 185kDa antibody
      • Myomesin 1 185kDa antibody
      • Myomesin 1, 185kDa antibody
      • Myomesin family member 1 antibody
      • Myomesin-1 antibody
      • Skelemin antibody
      see all

    Images

    • Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution + Human fetal heart lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 188 kDa
      Observed band size: 188 kDa


      Exposure time: 2 minutes


      This data was developed using ab205618, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      All lanes : Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 188 kDa
      Observed band size: 188 kDa


      Exposure time: 5 seconds


      This data was developed using ab205618, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      All lanes : Anti-MYOM1 antibody [EPR17322-9] (ab205618) at 1/5000 dilution

      Lane 1 : Rat heart tissue lysate
      Lane 2 : Rat brain tissue lysate
      Lane 3 : Rat kidney tissue lysate
      Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
      Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

      Predicted band size: 188 kDa
      Observed band size: 188 kDa


      Exposure time: 5 seconds


      This data was developed using ab205618, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      This data was developed using ab205618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human cardiac muscle tissue is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      This data was developed using ab205618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on human tonsil tissue is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      This data was developed using ab205618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      This data was developed using ab205618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)
      Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (ab251439)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251439? Please let us know so that we can cite the reference in this datasheet.

    ab251439 has not yet been referenced specifically in any publications.

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