Recombinant Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17322] to MYOM1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-MYOM1 antibody [EPR17322] - BSA and Azide free
See all MYOM1 primary antibodies -
Description
Rabbit monoclonal [EPR17322] to MYOM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab251338 is the carrier-free version of ab201228.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
EPR17322 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 188 kDa (predicted molecular weight: 188 kDa).
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 188 kDa (predicted molecular weight: 188 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent. -
Sequence similarities
Contains 5 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. - Information by UniProt
-
Database links
- Entrez Gene: 8736 Human
- Entrez Gene: 17929 Mouse
- Entrez Gene: 316740 Rat
- Omim: 603508 Human
- SwissProt: P52179 Human
- SwissProt: Q62234 Mouse
- Unigene: 464469 Human
-
Alternative names
- 190 kDa connectin-associated protein antibody
- 190 kDa titin-associated protein antibody
- EH myomesin antibody
see all
Images
-
All lanes : Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 188 kDa
Observed band size: 188 kDa
Exposure time: 1 minuteThis data was developed using ab201228, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution
Lane 1 : Rat heart lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat kidney lysate
Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 188 kDa
Observed band size: 188 kDa
Exposure time: 15 secondsThis data was developed using ab201228, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution + Human fetal heart tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 188 kDa
Observed band size: 188 kDa
Exposure time: 3 minutesThis data was developed using ab201228, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
This data was developed using ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
This data was developed using ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human tonsil tissue is a negative control for MYOM1. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
This data was developed using ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
This data was developed using ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab251338 has not yet been referenced specifically in any publications.