Overview

  • Product name

    Anti-Myosin 18B antibody
  • Description

    Rabbit polyclonal to Myosin 18B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 2420-2564 of Human (Q8IUG5).

  • Positive control

    • Human kidney tissue, A431 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • Function

    May be involved in intracellular trafficking of the muscle cell when in the cytoplasm, whereas entering the nucleus, may be involved in the regulation of muscle specific genes. May play a role in the control of tumor development and progression; restored MYO18B expression in lung cancer cells suppresses anchorage-independent growth.
  • Tissue specificity

    Selectively expressed in cardiac and skeletal muscles. Weakly expressed in testis, pancreas, placenta, prostate, lung and thymus.
  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Developmental stage

    Reaches an expression peak in the third day after induction and remains at similar level during successive myotubule maturation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > myofibril > sarcomere. Punctate pattern in undifferentiated myoblasts. Nuclear, on primary cardiomyocytes and adult muscle. A partial sarcomeric location was found in some cardiomyocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • BK125H2.1 antibody
    • DKFZp434C2422 antibody
    • DKFZp779C1668 antibody
    • MY18B_HUMAN antibody
    • MYO18B antibody
    • myosin 18B antibody
    • myosin XVIIIB antibody
    • Myosin-XVIIIb antibody
    see all

Images

  • ab121125 at 1/10 dilution staining Myosin 18B in Paraffin-emebedded Human kidney tissue by Immunohistochemistry. Note: moderate cytoplasmic positivity in renal tubules.
  • Immunofluorescent staining of human cell line A431 using ab121125 at a concentration of 4 µg/ml. Note: shows positivity in nucleus but not nucleoli & cytoplasm.

References

ab121125 has not yet been referenced specifically in any publications.

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