Overview

  • Product name

    Anti-Myosin 1G/HA2 antibody
    See all Myosin 1G/HA2 primary antibodies
  • Description

    Rabbit polyclonal to Myosin 1G/HA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Myosin 1G/HA2 aa 209-389.
    Database link: B0I1T2

  • Positive control

    • IHC-P: Human appendix and lymphoid tissue. ICC/IF: HepG2 cells.
  • General notes

     This product was previously labelled as Myosin 1G

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab234854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments.
    Precursor of the minor histocompatibility antigen HA-2. More generally, minor histocompatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and their expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocompatibility antigen in HLA-matched sibling marrow transplants. HA-2 is restricted to MHC class I HLA-A*0201.
  • Tissue specificity

    Specifically expressed in hematopoietic cells.
  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Cellular localization

    Cell membrane. Localization at the membrane is not highly dependent on phosphatidylinositol 4,5-bisphosphate levels. Released from the membrane in the presence of ATP. May be enriched in peripheral processes, such as microvilli or ruffles.
  • Information by UniProt
  • Database links

  • Alternative names

    • mHag HA-2 antibody
    • Minor histocompatibility antigen HA-2 antibody
    • Myo1g antibody
    • MYO1G_HUMAN antibody
    • myosin IG antibody
    see all

Images

  • Paraffin-embedded human lymphoid tissue stained for Myosin 1G/HA2 using ab234854 at 1/100 dilution in immunohistochemical analysis.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Myosin 1G/HA2 using ab234854 at 1/100 dilution in ICC/IF. Secondary antibody was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).

  • Paraffin-embedded human appendix tissue stained for Myosin 1G/HA2 using ab234854 at 1/100 dilution in immunohistochemical analysis.

References

ab234854 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab234854.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up