Key features and details
- Rabbit polyclonal to Myosin 1G/HA2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Myosin 1G/HA2 antibody
See all Myosin 1G/HA2 primary antibodies
DescriptionRabbit polyclonal to Myosin 1G/HA2
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Myosin 1G/HA2 aa 209-389.
Database link: B0I1T2
- IHC-P: Human appendix and lymphoid tissue. ICC/IF: HepG2 cells.
This product was previously labelled as Myosin 1G
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab234854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments.
Precursor of the minor histocompatibility antigen HA-2. More generally, minor histocompatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and their expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocompatibility antigen in HLA-matched sibling marrow transplants. HA-2 is restricted to MHC class I HLA-A*0201.
Tissue specificitySpecifically expressed in hematopoietic cells.
Sequence similaritiesContains 1 IQ domain.
Contains 1 myosin head-like domain.
Cellular localizationCell membrane. Localization at the membrane is not highly dependent on phosphatidylinositol 4,5-bisphosphate levels. Released from the membrane in the presence of ATP. May be enriched in peripheral processes, such as microvilli or ruffles.
- Information by UniProt
- mHag HA-2 antibody
- Minor histocompatibility antigen HA-2 antibody
- Myo1g antibody
Paraffin-embedded human lymphoid tissue stained for Myosin 1G/HA2 using ab234854 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Myosin 1G/HA2 using ab234854 at 1/100 dilution in ICC/IF. Secondary antibody was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
Paraffin-embedded human appendix tissue stained for Myosin 1G/HA2 using ab234854 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234854 has not yet been referenced specifically in any publications.