Key features and details
- Mouse monoclonal  to Myosin
- Suitable for: IHC-Fr, ICC, IHC-P, WB
- Reacts with: Chicken
- Isotype: IgM
Product nameAnti-Myosin antibody 
See all Myosin primary antibodies
DescriptionMouse monoclonal  to Myosin
SpecificityThis monoclonal antibody reacts in immunoblots specific with myosin and is specific to the 75kD of Myosin. Does not react with alpha-cardiac myosin. Does not react with the light-chain but with the last 75 kD of the myosin molecule. These 75 kD are located on a myosin-common site-chain.
Tested applicationsSuitable for: IHC-Fr, ICC, IHC-P, WBmore details
Species reactivityReacts with: Chicken
- Chicken muscle myomyofibrils.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityTissue culture supernatant
Primary antibody notesThis antibody, directed against myosin can be used for detection of myosin in frozen sections as well as in cultured cells. Applicable in paraffin sections only with microwave oven pretreatment. Also works in immunoblotting.
Light chain typeunknown
Our Abpromise guarantee covers the use of ab8070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.
Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris.
|WB||Use at an assay dependent concentration.|
Sequence similaritiesContains 1 IQ domain.
Contains 1 myosin head-like domain.
DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
- Information by UniProt
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
ab8070 has not yet been referenced specifically in any publications.