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    myosin-antibody-mac-147-ab51098.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Anti-Myosin antibody [MAC 147] (ab51098)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (8)

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Key features and details

  • Rat monoclonal [MAC 147] to Myosin
  • Reacts with: Drosophila melanogaster, Waterbug
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Myosin antibody [MAC 147]
    See all Myosin primary antibodies
  • Description

    Rat monoclonal [MAC 147] to Myosin
  • Host species

    Rat
  • Specificity

    Reacts with subfagment 2 of Lethocerus and Drosophila myosin
  • Species reactivity

    Reacts with: Drosophila melanogaster, Waterbug
  • Immunogen

    Flight muscle myosin purified from Lethocerus indicus (Waterbug)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    MAC 147
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

Target

  • Function

    Muscle contraction.
  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization

    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Target information above from: UniProt accession P12882 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MYH2 antibody
    • MYH3 antibody
    • MYH4 antibody
    • MYH6 antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • MYL1 antibody
    • MYL2 antibody
    • MYL3 antibody
    • MYL5 antibody
    • MYL6 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin 1 antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 1 skeletal muscle adult antibody
    • Myosin heavy chain 2 skeletal muscle adult antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain 3 skeletal muscle adult antibody
    • Myosin heavy chain 4 skeletal muscle adult antibody
    • Myosin heavy chain 6 skeletal muscle adult antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin heavy chain skeletal muscle adult 1 antibody
    • Myosin II antibody
    • Myosin light chain 1 alkali skeletal fast antibody
    • Myosin light chain 3 alkali ventricular skeletal slow antibody
    • Myosin light chain 4 alkali atrial embryonic antibody
    • Myosin light chain 5 regulatory antibody
    • Myosin light chain 6 alkali smooth muscle and non muscle antibody
    • Myosin-1 antibody
    • Myosin1 antibody
    • skeletal muscle antibody
    see all

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab51098? Please let us know so that we can cite the reference in this datasheet.

ab51098 has been referenced in 8 publications.

  • Di Cara F  et al. Distinct Roles for Peroxisomal Targeting Signal Receptors Pex5 and Pex7 in Drosophila. Genetics 211:141-149 (2019). PubMed: 30389805
  • Hessinger C  et al. The Drosophila Hox gene Ultrabithorax acts in both muscles and motoneurons to orchestrate formation of specific neuromuscular connections. Development 144:139-150 (2017). PubMed: 27913640
  • Molnár I  et al. DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila. PLoS Genet 10:e1004166 (2014). ICC/IF ; Drosophila melanogaster . PubMed: 24586196
  • Tarver MR  et al. Myosin Gene Expression and Protein Abundance in Different Castes of the Formosan Subterranean Termite (Coptotermes formosanus). Insects 3:1190-9 (2012). PubMed: 26466734
  • Hsu CY & Chan YP Identification and Localization of Proteins Associated with Biomineralization in the Iron Deposition Vesicles of Honeybees (Apis mellifera). PLoS One 6:e19088 (2011). WB ; Other species . PubMed: 21541330
  • Deng H  et al. Vestigial is required during late-stage muscle differentiation in Drosophila melanogaster embryos. Mol Biol Cell 21:3304-16 (2010). ICC/IF ; Drosophila melanogaster . PubMed: 20685961
  • Qiu F  et al. Myofilin, a protein in the thick filaments of insect muscle. J Cell Sci 118:1527-36 (2005). PubMed: 15769842
  • Fyrberg E  et al. Drosophila melanogaster troponin-T mutations engender three distinct syndromes of myofibrillar abnormalities. J Mol Biol 216:657-75 (1990). PubMed: 2124273

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin antibody [MAC 147]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Crab Cell (Mechanoreceptor neuron)
Specification
Mechanoreceptor neuron
Permeabilization
Yes - Tween
Fixative
Formaldehyde
Read More

Prof. Martin Mendelson

Verified customer

Submitted Jan 31 2014

Western blot abreview for Anti-Myosin antibody [MAC 147]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Termite Tissue lysate - whole (PBS extracted ground termite)
Loading amount
10 µg
Specification
PBS extracted ground termite
Treatment
methoprene
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE, DTT)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Mr. Chris Mattison

Verified customer

Submitted Sep 07 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin antibody [MAC 147]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (Body Wall Muslce)
Specification
Body Wall Muslce
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Dr. Julie Ni

Verified customer

Submitted Nov 26 2010

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