Anti-Myosin antibody [MAC 147] (ab51098)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Anti-Myosin antibody [MAC 147]
See all Myosin primary antibodies -
Description
Rat monoclonal [MAC 147] to Myosin -
Host species
Rat -
Specificity
Reacts with subfagment 2 of Lethocerus and Drosophila myosin -
Tested applications
Suitable for: ICC/IF, Electron Microscopy, WBmore details -
Species reactivity
Reacts with: Drosophila melanogaster, Waterbug -
Immunogen
Flight muscle myosin purified from Lethocerus indicus (Waterbug)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MAC 147 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab51098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent dilution. | |
| Electron Microscopy | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. Predicted molecular weight: 12 kDa. |
Target
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Function
Muscle contraction. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localization
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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Alternative names
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Myosin antibody [MAC 147] (ab51098)](https://www.abcam.com/ps/products/51/ab51098/Images/ab51098-63003-anti-myosin-antibody-mac-147-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Myosin antibody [MAC 147] (ab51098)This image is courtesy of an Abreview by Julie Ni.ab51098 staining Myosin in Drosophila Melanogaster body wall muscle cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed prior to blocking in 1% BSA for 10 minutes at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 16 hours at 4°C. The secondary antibody was an undiluted Alexa Fluor® 647-conjugated Donkey anti-Rat polyclonal.
References
This product has been referenced in:
- Hessinger C et al. The Drosophila Hox gene Ultrabithorax acts in both muscles and motoneurons to orchestrate formation of specific neuromuscular connections. Development 144:139-150 (2017). Read more (PubMed: 27913640) »
- Molnár I et al. DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila. PLoS Genet 10:e1004166 (2014). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 24586196) »
