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Recombinant

Recombinant Anti-Myosin Heavy Chain/MHC antibody [A4.1025] (ab37484)

Submit a review Submit a question References (1)
Western blot - Anti-Myosin antibody [A4.1025] (ab37484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
  • Immunohistochemistry (Frozen sections) - Anti-Myosin antibody [A4.1025] (ab37484)
  • Immunocytochemistry - Anti-Myosin antibody [A4.1025] (ab37484)
  • Western blot - Anti-Myosin antibody [A4.1025] (ab37484)
  • Western blot - Anti-Myosin antibody [A4.1025] (ab37484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
  • Immunohistochemistry (Frozen sections) - Anti-Myosin antibody [A4.1025] (ab37484)
  • Anti-Myosin antibody [A4.1025] (ab37484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [A4.1025] to Myosin
  • Suitable for: IHC-P, IHC-Fr, ICC, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Myosin Heavy Chain/MHC antibody [A4.1025]
    See all Myosin primary antibodies

  • Description

    Mouse monoclonal [A4.1025] to Myosin

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human, mouse and rat skeletal muscle tissue lysates; Differentiated C2C12 whole cell lysate IHC-P: Human skeletal and cardiac muscle tissue. IHC-Fr: Mouse and rat skeletal muscle tissue. ICC: Differentiated C2C12 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    A4.1025

  • Isotype

    IgG2a

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab37484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 0.807 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use a concentration of 16.14 µg/ml.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
ICC
Use a concentration of 3.228 µg/ml.
WB
Use a concentration of 0.1614 - 0.807 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
Notes
IHC-P
Use a concentration of 0.807 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use a concentration of 16.14 µg/ml.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
ICC
Use a concentration of 3.228 µg/ml.
WB
Use a concentration of 0.1614 - 0.807 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).

Target

Images

  • Western blot - Anti-Myosin antibody [A4.1025] (ab37484)
    Western blot - Anti-Myosin antibody [A4.1025] (ab37484)
    Anti-Myosin Heavy Chain/MHC antibody [A4.1025] (ab37484) at 0.1614 µg/ml + Human skeletal muscle tissue lysate at 20 µg

    Secondary
    Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 223 kDa
    Observed band size: 223 kDa


    Exposure time: 3 seconds


    The extra bands under 223KDa were mainly caused by degradation (PMID: 3295257).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Myosin antibody [A4.1025] (ab37484)
    Immunohistochemistry (Frozen sections) - Anti-Myosin antibody [A4.1025] (ab37484)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 µg/ml followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry - Anti-Myosin antibody [A4.1025] (ab37484)
    Immunocytochemistry - Anti-Myosin antibody [A4.1025] (ab37484)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Myosin (MYH1/2) with ab37484 at 3.228 µg/ml, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2µg/ml) (Green). Confocal image showing cytoplasmic staining in differentiated (treated) C2C12 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (2.5µg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4µg/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2µg/ml).

  • Western blot - Anti-Myosin antibody [A4.1025] (ab37484)
    Western blot - Anti-Myosin antibody [A4.1025] (ab37484)
    All lanes : Anti-Myosin Heavy Chain/MHC antibody [A4.1025] (ab37484) at 0.807 µg/ml

    Lane 1 : Mouse skeletal muscle tissue lysate
    Lane 2 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 223 kDa
    Observed band size: 223 kDa



    Exposure times: Lane 1: 3 secs; Lane 2: 1 sec.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Myosin antibody [A4.1025] (ab37484)
    Western blot - Anti-Myosin antibody [A4.1025] (ab37484)
    All lanes : Anti-Myosin Heavy Chain/MHC antibody [A4.1025] (ab37484) at 0.807 µg/ml

    Lane 1 : C2C12 (mouse myoblasts) whole cell lysate
    Lane 2 : Differentiated C2C12 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 223 kDa
    Observed band size: 223 kDa


    Exposure time: 3 seconds


    The expression profile observed is consistent with what has been described in the literature (PMID: 26010876).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cardiac muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat  skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Myosin antibody [A4.1025] (ab37484)
    Immunohistochemistry (Frozen sections) - Anti-Myosin antibody [A4.1025] (ab37484)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 µg/ml followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-Myosin antibody [A4.1025] (ab37484)
    Anti-Myosin antibody [A4.1025] (ab37484)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab37484? Please let us know so that we can cite the reference in this datasheet.

ab37484 has been referenced in 1 publication.

  • Hughes SM & Blau HM Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell 68:659-71 (1992). PubMed: 1531450

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