Anti-Myosin IXA antibody (ab241200)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Myosin IXA antibody
See all Myosin IXA primary antibodies -
Description
Rabbit polyclonal to Myosin IXA -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human Myosin IXA aa 2350-2400. The exact sequence is proprietary.
Database link: B2RTY4 -
Positive control
- IP: HeLa cell lysate.
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General notes
ab241200 has not performed satisfactorily when used for WB of Myosin IXA in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Myosin IXA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Myosin IXA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. 8 - 15 µl/mg lysate |
Target
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Function
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Regulates Rho activity in neurons, has a role in the regulation of neuronal morphology and function. -
Tissue specificity
Found to be expressed in testis and placenta, and at lower levels in all the examined tissues with the exception of liver. Isoform 5 was found in leukocytes but not in brain, retina or testis. -
Sequence similarities
Contains 5 IQ domains.
Contains 2 myosin head-like domains.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 Ras-associating domain.
Contains 1 Rho-GAP domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4649 Human
- Entrez Gene: 270163 Mouse
- Omim: 604875 Human
- SwissProt: B2RTY4 Human
- SwissProt: Q8C170 Mouse
- Unigene: 546268 Human
- Unigene: 249545 Mouse
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Alternative names
- 4732465J09Rik antibody
- C130068I12Rik antibody
- C230003M11 antibody
see all
Images
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Immunoprecipitation - Anti-Myosin IXA antibody (ab241200)Myosin IXA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241200 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab241200 at 1/1000 dilution.
Lane 1: ab241200 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
Datasheets and documents
References
ab241200 has not yet been referenced specifically in any publications.
