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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Anti-Myosin IXA antibody (ab241200)

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Immunoprecipitation - Anti-Myosin IXA antibody (ab241200)

    Key features and details

    • Rabbit polyclonal to Myosin IXA
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Myosin IXA protein (ab158945)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Myosin IXA antibody
      See all Myosin IXA primary antibodies
    • Description

      Rabbit polyclonal to Myosin IXA
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Synthetic peptide within Human Myosin IXA aa 2350-2400. The exact sequence is proprietary.
      Database link: B2RTY4

    • Positive control

      • IP: HeLa cell lysate.
    • General notes

      ab241200 has not performed satisfactorily when used for WB of Myosin IXA in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Myosin IXA.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: Tris buffered saline, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antibody was affinity purified using an epitope specific to Myosin IXA immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Motor Proteins
      • Myosin

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
    • Recombinant Protein

      • Recombinant Human Myosin IXA protein (ab158945)

    Applications

    Our Abpromise guarantee covers the use of ab241200 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at an assay dependent concentration.

    8 - 15 µl/mg lysate

    Target

    • Function

      Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Regulates Rho activity in neurons, has a role in the regulation of neuronal morphology and function.
    • Tissue specificity

      Found to be expressed in testis and placenta, and at lower levels in all the examined tissues with the exception of liver. Isoform 5 was found in leukocytes but not in brain, retina or testis.
    • Sequence similarities

      Contains 5 IQ domains.
      Contains 2 myosin head-like domains.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 Ras-associating domain.
      Contains 1 Rho-GAP domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession B2RTY4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4649 Human
      • Entrez Gene: 270163 Mouse
      • Omim: 604875 Human
      • SwissProt: B2RTY4 Human
      • SwissProt: Q8C170 Mouse
      • Unigene: 546268 Human
      • Unigene: 249545 Mouse
      • Alternative names

        • 4732465J09Rik antibody
        • C130068I12Rik antibody
        • C230003M11 antibody
        • FLJ11061 antibody
        • FLJ13244 antibody
        • MGC71859 antibody
        • Myo9a antibody
        • MYO9A variant protein antibody
        • MYO9A_HUMAN antibody
        • Myosin-IXa antibody
        • MYR7 antibody
        • Unconventional myosin 9a antibody
        • Unconventional myosin-9a antibody
        see all

      Images

      • Immunoprecipitation - Anti-Myosin IXA antibody (ab241200)
        Immunoprecipitation - Anti-Myosin IXA antibody (ab241200)

        Myosin IXA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241200 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab241200 at 1/1000 dilution.

        Lane 1: ab241200 IP in HeLa whole cell lysate.
        Lane 2: Control IgG IP in HeLa whole cell lysate.

        Detection: Chemiluminescence with exposure time of 30 seconds.

        Lysates prepared in NETN buffer.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab241200? Please let us know so that we can cite the reference in this datasheet.

      ab241200 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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