Anti-Myosin IXA antibody (ab241200)
Key features and details
- Rabbit polyclonal to Myosin IXA
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Myosin IXA antibody
See all Myosin IXA primary antibodies -
Description
Rabbit polyclonal to Myosin IXA -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human Myosin IXA aa 2350-2400. The exact sequence is proprietary.
Database link: B2RTY4 -
Positive control
- IP: HeLa cell lysate.
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General notes
ab241200 has not performed satisfactorily when used for WB of Myosin IXA in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Myosin IXA.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Myosin IXA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. 8 - 15 µl/mg lysate |
Target
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Function
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Regulates Rho activity in neurons, has a role in the regulation of neuronal morphology and function. -
Tissue specificity
Found to be expressed in testis and placenta, and at lower levels in all the examined tissues with the exception of liver. Isoform 5 was found in leukocytes but not in brain, retina or testis. -
Sequence similarities
Contains 5 IQ domains.
Contains 2 myosin head-like domains.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 Ras-associating domain.
Contains 1 Rho-GAP domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4649 Human
- Entrez Gene: 270163 Mouse
- Omim: 604875 Human
- SwissProt: B2RTY4 Human
- SwissProt: Q8C170 Mouse
- Unigene: 546268 Human
- Unigene: 249545 Mouse
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Alternative names
- 4732465J09Rik antibody
- C130068I12Rik antibody
- C230003M11 antibody
see all
Images
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Immunoprecipitation - Anti-Myosin IXA antibody (ab241200)
Myosin IXA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241200 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab241200 at 1/1000 dilution.
Lane 1: ab241200 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241200 has not yet been referenced specifically in any publications.
