Key features and details
- Rabbit polyclonal to Myosin Light Chain 2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Product nameAnti-Myosin Light Chain 2 antibody
See all Myosin Light Chain 2 primary antibodies
DescriptionRabbit polyclonal to Myosin Light Chain 2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Rabbit, Cow
Recombinant fragment (His-tag) corresponding to Human Myosin Light Chain 2 aa 2-159. (Expressed in E.coli).
APKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRD TFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILN AFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAFPPDVTGNLDY KNLVHII
Database link: P10916
- IHC-P: Human liver, liver cancer, esophagus and rectum cancer tissue. WB: Human serum; Rat heart, skin and skeletal muscle lysate; Mouse heart lysate; Pig heart lysate; Recombinant human Myosin Light Chain 2 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233152 in the following tested applications.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
Involvement in diseaseDefects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
Sequence similaritiesContains 3 EF-hand domains.
modificationsN-terminus is methylated by METTL11A/NTM1.
- Information by UniProt
- Cardiac myosin light chain-2 antibody
- Cardiac ventricular myosin light chain 2 antibody
- CMH10 antibody
Formalin-fixed, paraffin-embedded human rectum cancer tissue stained for Myosin Light Chain 2 using ab233152 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human esophagus tissue stained for Myosin Light Chain 2 using ab233152 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Myosin Light Chain 2 using ab233152 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for Myosin Light Chain 2 using ab233152 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-Myosin Light Chain 2 antibody (ab233152) at 3 µg/ml
Lane 1 : Human serum
Lane 2 : Rat heart lysate
Lane 3 : Rat skin lysate
Lane 4 : Rat skeletal muscle lysate
Lane 5 : Mouse heart lysate
Lane 6 : Pig heart lysate
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Anti-Myosin Light Chain 2 antibody (ab233152) at 2 µg/ml + Recombinant human Myosin Light Chain 2 protein
ab233152 has not yet been referenced specifically in any publications.