Anti-Myosin Light Chain 2 antibody (ab48003)

Rabbit polyclonal Myosin Light Chain 2 antibody. Validated in WB, ELISA, IHC, ICC/IF and tested in Rat. Cited in 9 publication(s). Independently reviewed in 1 review(s).


  • Product name

    Anti-Myosin Light Chain 2 antibody
    See all Myosin Light Chain 2 primary antibodies
  • Description

    Rabbit polyclonal to Myosin Light Chain 2
  • Host species

  • Specificity

    ab48003 is directed against the regulatory light chain of smooth and non muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein.
  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 12/27 of Human Myosin Light Chain 2

  • Positive control

    • Recombinant Human Myosin Light Chain 2 protein (ab79185) can be used as a positive control in WB. rat aorta



Our Abpromise guarantee covers the use of ab48003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/20000.
WB 1/500 - 1/2000. Predicted molecular weight: 19 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 23704920
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Involvement in disease

    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • Sequence similarities

    Contains 3 EF-hand domains.
  • Post-translational

    N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cardiac myosin light chain-2 antibody
    • Cardiac ventricular myosin light chain 2 antibody
    • CMH10 antibody
    • MLC 2v antibody
    • MLC-2 antibody
    • MLC-2v antibody
    • MLC2 antibody
    • MLRV_HUMAN antibody
    • MYL 2 antibody
    • MYL2 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin light polypeptide 2 regulatory cardiac slow antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
    • Regulatory light chain of myosin antibody
    • RLC of myosin antibody
    • Slow cardiac myosin regulatory light chain 2 antibody
    • ventricular/cardiac muscle isoform antibody
    see all


  • All lanes : Anti-Myosin Light Chain 2 antibody (ab48003) at 1/600 dilution

    Lane 1 : Vascular myosin (rat aorta), loaded with 35 µg of lysate
    Lane 2 : Cardiac myosin (mouse heart, loaded with 35 µg of lysate

    All lanes : 1:10,000 dilution of
    IRDye800™ conjugated Gt-a-Rabbit IgG [H&L]

    Predicted band size: 19 kDa
    Observed band size: 19,24 kDa
    why is the actual band size different from the predicted?

    Arrowheads indicate the detection of both monophosphorylated (upper) and unphosphorylated (lower) forms of the protein.
  • ab48003 (2µg/ml) staining Myosin Light Chain 2 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cell cytoplasm.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


This product has been referenced in:

  • Patrinostro X  et al. Relative importance of ßcyto- and ?cyto-actin in primary mouse embryonic fibroblasts. Mol Biol Cell 28:771-782 (2017). Read more (PubMed: 28077619) »
  • Li L  et al. Myosin light chain kinase facilitates endocytosis of synaptic vesicles at hippocampal boutons. J Neurochem 138:60-73 (2016). Read more (PubMed: 27062289) »
See all 9 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Thank you for your reply.

I can confirm that the immunogen for ab48003 was a synthetic peptide corresponding to amino acids 12 to 27 of Human Myosin Light Chain 2 (GANSNVFSMFEQTQIQ).

The antibody therefore binds to this region.

I can only confirm that the antibody bindsboth unphosphorylated and monophosphorylated forms of the protein. We assume this binding is equal, but this has not been tested experimentally.

I hope this information is helpful and wish you good luck with your research.

Read More
Immunocytochemistry/ Immunofluorescence
Human Cell (Cardiac cells)
Yes - 0.05% TritionX100
Cardiac cells
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 16 2016


We recommend use of a 5% non fat dry milk/TBS blocking and incubation solution. We also recommend a starting dilution for the antibody within the range 1/500 - 1/2000. This is just a suggested starting range for the product. The optimal antibody dilution may ultimately be outside of this range and should be determined in the lab.

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I have issued a free of charge replacement for one vial of ab92721. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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DISCOUNT CODE: *** Expiration date: April 6th, 2012 I am very pleased to hear you would like to accept our offer and test ab48003 in ICC on mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC on mouse cells and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here:  

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