• Product name
    Anti-Myosin Light Chain 2 antibody
    See all Myosin Light Chain 2 primary antibodies
  • Description
    Rabbit polyclonal to Myosin Light Chain 2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Myosin Light Chain 2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab88166.)

  • Positive control
    • Recombinant Human Myosin Light Chain 2 protein (ab117178) can be used as a positive control in WB. This antibody gave a positive signal in the following tissues: Human Heart; Human Skeletal Muscle; Mouse Heart; Rat Heart



Our Abpromise guarantee covers the use of ab79935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/140.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.


  • Involvement in disease
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • Sequence similarities
    Contains 3 EF-hand domains.
  • Post-translational
    N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac myosin light chain-2 antibody
    • Cardiac ventricular myosin light chain 2 antibody
    • CMH10 antibody
    • MLC 2v antibody
    • MLC-2 antibody
    • MLC-2v antibody
    • MLC2 antibody
    • MLRV_HUMAN antibody
    • MYL 2 antibody
    • MYL2 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin light polypeptide 2 regulatory cardiac slow antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
    • Regulatory light chain of myosin antibody
    • RLC of myosin antibody
    • Slow cardiac myosin regulatory light chain 2 antibody
    • ventricular/cardiac muscle isoform antibody
    see all


  • ab79935 staining Myosin Light Chain 2 in Mouse embyronic (e18.5) cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% Serum for 1 hour 30 minutes at 20°C. Samples were incubated with primary antibody (1/140 in 1% goat serum + 10% BSA + 0.1% Triton X-100 in PBS) for 16 hours at 4°C. An AlexaFluor® 594-conjugated Goat anti-rabbit IgG (H+L) (1/200) was used as the secondary antibody.

    See Abreview

  • Myosin Light Chain 2 was immunoprecipitated using 0.5mg Mouse Heart tissue, 5µg of Rabbit polyclonal to Myosin Light Chain 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79935.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 19kDa; Myosin Light Chain 2

  • IHC image of ab79935 staining in Mouse Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79935, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-Myosin Light Chain 2 antibody (ab79935) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa
    Observed band size: 19 kDa
    Additional bands at: 17 kDa, 8 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 15 minutes


This product has been referenced in:
  • Huang W  et al. Heterogeneity of adult masseter muscle satellite cells with cardiomyocyte differentiation potential. Exp Cell Res N/A:N/A (2018). Read more (PubMed: 29842877) »
  • Lee JH  et al. Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations. Cell Stem Cell 21:179-194.e4 (2017). Flow Cyt . Read more (PubMed: 28777944) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Flow Cytometry
Human Cell (iPS derived cardiomyocytes)
Yes - Saponin
Gating Strategy
Gate was created using the same cells maked only with secondary antibody.
iPS derived cardiomyocytes
Cell harvesting/tissue preparation method: Cells were haversted after trypsin digestion for 10 min and blocked with 10% FBS. After wash with HBSS, cells were fixed with 1% PFA.
Sample buffer: Cells were kept in HBSS until blocking with HBSS + 0,2% saponin + 3% dry-milk. Washing solution consisted in HBSS + 2% saponin+ 1% BSA.

Dr. Diogo Biagi

Verified customer

Submitted Apr 04 2019


Unfortunately, this is a customer review and both the data and the protocol information comes from the customer themselves. We therefore do not have any additional information regarding this image and what the green fluorescence corresponds to.

Please note that all our products are covered by our one year guarantee and should a product not work as stated on the datasheet, we would be happy to replace or refund the antibody.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (Embryonic (E18.5) cardiomyocyte)
Embryonic (E18.5) cardiomyocyte
Yes - 0.5% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 18 2012


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