Anti-Myosin Light Chain 2 antibody (ab79935)
- Datasheet
- References (12)
- Protocols
Overview
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Product name
Anti-Myosin Light Chain 2 antibody
See all Myosin Light Chain 2 primary antibodies -
Description
Rabbit polyclonal to Myosin Light Chain 2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human Myosin Light Chain 2 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab88166) -
Positive control
- Recombinant Human Myosin Light Chain 2 protein (ab117178) can be used as a positive control in WB. This antibody gave a positive signal in the following tissues: Human Heart; Human Skeletal Muscle; Mouse Heart; Rat Heart
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab79935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/140. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| IP | Use a concentration of 5 µg/ml. |
Target
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Involvement in disease
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
Sequence similarities
Contains 3 EF-hand domains. -
Post-translational
modificationsN-terminus is methylated by METTL11A/NTM1. - Information by UniProt
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Database links
- Entrez Gene: 505519 Cow
- Entrez Gene: 403614 Dog
- Entrez Gene: 4633 Human
- Entrez Gene: 17906 Mouse
- Entrez Gene: 363925 Rat
- Omim: 160781 Human
- SwissProt: Q3SZE5 Cow
- SwissProt: P10916 Human
see all -
Alternative names
- Cardiac myosin light chain-2 antibody
- Cardiac ventricular myosin light chain 2 antibody
- CMH10 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Myosin Light Chain 2 antibody (ab79935)This image is courtesy of an anonymous Abreviewab79935 staining Myosin Light Chain 2 in Mouse embyronic (e18.5) cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% Serum for 1 hour 30 minutes at 20°C. Samples were incubated with primary antibody (1/140 in 1% goat serum + 10% BSA + 0.1% Triton X-100 in PBS) for 16 hours at 4°C. An AlexaFluor® 594-conjugated Goat anti-rabbit IgG (H+L) (1/200) was used as the secondary antibody. -
Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (ab79935)Myosin Light Chain 2 was immunoprecipitated using 0.5mg Mouse Heart tissue, 5µg of Rabbit polyclonal to Myosin Light Chain 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79935.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 19kDa; Myosin Light Chain 2
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (ab79935)IHC image of ab79935 staining in Mouse Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79935, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Myosin Light Chain 2 antibody (ab79935)All lanes : Anti-Myosin Light Chain 2 antibody (ab79935) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Additional bands at: 17 kDa, 8 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
Protocols
References
This product has been referenced in:
- Wang K et al. Differential roles of insulin like growth factor 1 receptor and insulin receptor during embryonic heart development. BMC Dev Biol 19:5 (2019). Read more (PubMed: 30909860) »
- Goldfracht I et al. Engineered heart tissue models from hiPSC-derived cardiomyocytes and cardiac ECM for disease modeling and drug testing applications. Acta Biomater 92:145-159 (2019). Read more (PubMed: 31075518) »
