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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Anti-Myosin Light Chain 2 antibody (ab79935)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (17)

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Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (ab79935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (ab79935)
  • Western blot - Anti-Myosin Light Chain 2 antibody (ab79935)

Key features and details

  • Rabbit polyclonal to Myosin Light Chain 2
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Primary
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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human Myosin Light Chain 2 protein (ab79185)

View more associated products

Overview

  • Product name

    Anti-Myosin Light Chain 2 antibody
    See all Myosin Light Chain 2 primary antibodies
  • Description

    Rabbit polyclonal to Myosin Light Chain 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Myosin Light Chain 2 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab88166)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Myosins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Myosin Light Chain 2 protein (ab79185)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P
Use a concentration of 5 µg/ml.
IP
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P
Use a concentration of 5 µg/ml.
IP
Use a concentration of 5 µg/ml.

Target

  • Involvement in disease

    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • Sequence similarities

    Contains 3 EF-hand domains.
  • Post-translational
    modifications

    N-terminus is methylated by METTL11A/NTM1.
  • Target information above from: UniProt accession P10916 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 505519 Cow
    • Entrez Gene: 403614 Dog
    • Entrez Gene: 4633 Human
    • Entrez Gene: 17906 Mouse
    • Entrez Gene: 363925 Rat
    • Omim: 160781 Human
    • SwissProt: Q3SZE5 Cow
    • SwissProt: P10916 Human
    • SwissProt: P51667 Mouse
    • SwissProt: P08733 Rat
    • Unigene: 75535 Human
    • Unigene: 1529 Mouse
    • Unigene: 37176 Rat
    • Unigene: 6534 Rat
    see all
  • Alternative names

    • Cardiac myosin light chain-2 antibody
    • Cardiac ventricular myosin light chain 2 antibody
    • CMH10 antibody
    • MLC 2v antibody
    • MLC-2 antibody
    • MLC-2v antibody
    • MLC2 antibody
    • MLRV_HUMAN antibody
    • MYL 2 antibody
    • MYL2 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin light polypeptide 2 regulatory cardiac slow antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
    • Regulatory light chain of myosin antibody
    • RLC of myosin antibody
    • Slow cardiac myosin regulatory light chain 2 antibody
    • ventricular/cardiac muscle isoform antibody
    see all

Images

  • Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (ab79935)
    Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (ab79935)

    Myosin Light Chain 2 was immunoprecipitated using 0.5mg Mouse Heart tissue, 5µg of Rabbit polyclonal to Myosin Light Chain 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79935.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 19kDa; Myosin Light Chain 2

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (ab79935)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (ab79935)

    IHC image of Myosin light chain 2 antibody staining in a section of formalin-fixed paraffin-embedded normal mouse heart performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab79935, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Western blot - Anti-Myosin Light Chain 2 antibody (ab79935)
    Western blot - Anti-Myosin Light Chain 2 antibody (ab79935)
    All lanes : Anti-Myosin Light Chain 2 antibody (ab79935) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa
    Observed band size: 19 kDa
    Additional bands at: 17 kDa, 8 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 15 minutes

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (17)

Publishing research using ab79935? Please let us know so that we can cite the reference in this datasheet.

ab79935 has been referenced in 17 publications.

  • Goldfracht I  et al. Generating ring-shaped engineered heart tissues from ventricular and atrial human pluripotent stem cell-derived cardiomyocytes. Nat Commun 11:75 (2020). PubMed: 31911598
  • Shen H  et al. Mononuclear diploid cardiomyocytes support neonatal mouse heart regeneration in response to paracrine IGF2 signaling. Elife 9:N/A (2020). PubMed: 32167474
  • Testa G  et al. Bmi1 inhibitor PTC-209 promotes Chemically-induced Direct Cardiac Reprogramming of cardiac fibroblasts into cardiomyocytes. Sci Rep 10:7129 (2020). PubMed: 32346096
  • Choi S  et al. Regulation of endothelial barrier integrity by redox-dependent nitric oxide signaling: Implication in traumatic and inflammatory brain injuries. Nitric Oxide 83:51-64 (2019). PubMed: 30590116
  • Hori A  et al. Prominin-1 Modulates Rho/ROCK-Mediated Membrane Morphology and Calcium-Dependent Intracellular Chloride Flux. Sci Rep 9:15911 (2019). PubMed: 31685837
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-3 of 3 Abreviews or Q&A

Flow Cytometry abreview for Anti-Myosin Light Chain 2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (iPS derived cardiomyocytes)
Permeabilization
Yes - Saponin
Gating Strategy
Gate was created using the same cells maked only with secondary antibody.
Specification
iPS derived cardiomyocytes
Preparation
Cell harvesting/tissue preparation method: Cells were haversted after trypsin digestion for 10 min and blocked with 10% FBS. After wash with HBSS, cells were fixed with 1% PFA.
Sample buffer: Cells were kept in HBSS until blocking with HBSS + 0,2% saponin + 3% dry-milk. Washing solution consisted in HBSS + 2% saponin+ 1% BSA.
Fixation
Paraformaldehyde
Read More

Dr. Diogo Biagi

Verified customer

Submitted Apr 04 2019

Question

Would you be able to tell me what the green fluorescence is targeting in this picture:

https://www.abcam.com/myosin-light-chain-2-antibody-ab79935.html/reviews/30309

It says the secondary antibody against the primary MLC-2V antibody is alexafluor 594, which is red, so I’m guessing the red fluorescence in the image is the MLC-2V. It doesn’t say what the sarcomeric green fluorescence is though?

Read More

Abcam community

Verified customer

Asked on Aug 29 2014

Answer

Unfortunately, this is a customer review and both the data and the protocol information comes from the customer themselves. We therefore do not have any additional information regarding this image and what the green fluorescence corresponds to.

Please note that all our products are covered by our one year guarantee and should a product not work as stated on the datasheet, we would be happy to replace or refund the antibody.

Read More

Elisa Thomas

Abcam Scientific Support

Answered on Aug 29 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin Light Chain 2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic (E18.5) cardiomyocyte)
Specification
Embryonic (E18.5) cardiomyocyte
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jun 18 2012

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