Recombinant
RabMAb

Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

Abreviews (5)Q&A (7)Specific References (11)

Overview

  • Product name
    Anti-Myosin Light Chain 2 antibody [EPR3741]
    See all Myosin Light Chain 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3741] to Myosin Light Chain 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Myosin Light Chain 2 aa 150-250. The exact sequence is proprietary.

  • Positive control
    • WB: Fetal heart, fetal skeletal muscle, Human heart, mouse heart and rat heart lysates IHC-P: Human heart muscle
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 23365102
WB 1/5000. Predicted molecular weight: 19 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Involvement in disease
      Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
    • Sequence similarities
      Contains 3 EF-hand domains.
    • Post-translational
      modifications
      N-terminus is methylated by METTL11A/NTM1.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Cardiac myosin light chain-2 antibody
      • Cardiac ventricular myosin light chain 2 antibody
      • CMH10 antibody
      • MLC 2v antibody
      • MLC-2 antibody
      • MLC-2v antibody
      • MLC2 antibody
      • MLRV_HUMAN antibody
      • MYL 2 antibody
      • MYL2 antibody
      • Myosin light chain 2 regulatory cardiac slow antibody
      • Myosin light polypeptide 2 regulatory cardiac slow antibody
      • Myosin regulatory light chain 2 antibody
      • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
      • Regulatory light chain of myosin antibody
      • RLC of myosin antibody
      • Slow cardiac myosin regulatory light chain 2 antibody
      • ventricular/cardiac muscle isoform antibody
      see all

    Images

    • Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : Fetal skeletal muscle lysate
      Lane 3 : Human heart lysate
      Lane 4 : Mouse heart lysate
      Lane 5 : Rat heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 19 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      ab92721, at a 1/100 dilution, staining Myosin Light Chain 2 in paraffin embedded Human heart muscle by Immunohistochemistry.
    • Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      ab92721 showing positive staining in Heart muscle tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      ab92721 showing positive staining in Skeletal muscle tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      ab92721 showing negative staining in Normal tonsil tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      ab92721 showing negative staining in Normal brain tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      ab92721 showing negative staining in Normal liver tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      ab92721 showing negative staining in Normal kidney tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      ab92721 showing negative staining in Normal breast tissue.

    • Other - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Other - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Xia K  et al. Effect of QSKL on MAPK and RhoA Pathways in a Rat Model of Heart Failure. Evid Based Complement Alternat Med 2017:3903898 (2017). WB ; Rat . Read more (PubMed: 28484504) »
    • Kudová J  et al. HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells. PLoS One 11:e0158358 (2016). Read more (PubMed: 27355368) »

    See all 11 Publications for this product

    Publishing research using ab92721? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    12 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin Light Chain 2 antibody [EPR3741]

     Poor
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Oct 27 2015

    Western blot abreview for Anti-Myosin Light Chain 2 antibody [EPR3741]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Ileon)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    Ileon
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Sep 30 2015

    Western blot

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Specification
    Skeletal muscle
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 22 2013

    Western blot

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Mouse Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 22 2013

    Western blot

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Human Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 22 2013

    I need to order antibodies for MYL7 ((ab92721) and MYL2 (ab137063). While going through the datasheets, I've seen IHC images of these antibodies but no reference is given for them. Can you provide us with the references for those images. Also, as we ar...

    Read More

    Thank you for contacting us with your questions.

    The IHC images on the datasheets for ab92721 and ab137063 were produced in the Epitomics lab, and they weren't taken from publications so there are no references. Ab92721 was used in one public...

    Read More

    Thank you very much for helping me solving this problem.
    The antibody for troponin T that I worked before was ab8295, probably works better with my type of samples.
    I need antibodies that prove cardiac differentiation, that is why I bought ca...

    Read More

    Are you interested in an antibody against a different cardiac marker such as GATA4? The antibody ab86371 looks good.

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=86371).

    The antibody ab8295, which y...

    Read More

    I bought 2 antobodies from your company and I am not able to get signal when I perform western-blotting under the conditions advised by you. I tried to increase also the concentration of antibody and I was unable to have a result. I am sure that is not...

    Read More

    Thank you for bringing this to our attention. If I cannot offer a useful suggestion to improve your results, and if the antibodies are covered by our guarantee, I will send replacements.

    I will first need a few details of your experiment and...

    Read More

    I have used ab48003 to stain primary mouse cell cultures of cell types I expect to be positive and cell types I expect to be negative for for myosin light chain 2. However all samples stain equally well with this antibody, leading me to think it is rea...

    Read More

    I have issued a free of charge replacement for one vial of ab92721. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abprom...

    Read More

    What is the WB protocol used for this antibody?

    Thank you for contacting Abcam regarding ab92721 and ab89594. I have received the following protocol information from the laboratory: Western Blotting Protocol Solutions and Reagents 2X Laemmli Sample Buffer: 62.5 mM Tris-HCl (pH 6.8), ...

    Read More

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