Recombinant Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3741] to Myosin Light Chain 2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Myosin Light Chain 2 antibody [EPR3741]
See all Myosin Light Chain 2 primary antibodies -
Description
Rabbit monoclonal [EPR3741] to Myosin Light Chain 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: human heart, human skeletal muscle, mouse heart, rat heart lysates IHC: human cardiac muscle, mouse cardiac muscle, rat cardiac muscle
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General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.78 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3741 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/10000. Predicted molecular weight: 19 kDa.
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IHC-P |
1/600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended. |
Notes |
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WB
1/10000. Predicted molecular weight: 19 kDa. |
IHC-P
1/600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended. |
Target
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Involvement in disease
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
Sequence similarities
Contains 3 EF-hand domains. -
Post-translational
modificationsN-terminus is methylated by METTL11A/NTM1. - Information by UniProt
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Database links
- Entrez Gene: 4633 Human
- Entrez Gene: 17906 Mouse
- Entrez Gene: 363925 Rat
- Omim: 160781 Human
- SwissProt: P10916 Human
- SwissProt: P51667 Mouse
- SwissProt: P08733 Rat
- Unigene: 75535 Human
see all -
Alternative names
- Cardiac myosin light chain-2 antibody
- Cardiac ventricular myosin light chain 2 antibody
- CMH10 antibody
see all
Images
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All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution (Purified)
Lane 1 : Human heart lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : Mouse heart lysate
Lane 4 : Rat heart lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling Myosin Light Chain 2 with Purified ab92721 at 1/600 dilution (0.16 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Myosin Light Chain 2 with Purified ab92721 at 1/600 dilution (0.16 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Myosin Light Chain 2 with Purified ab92721 at 1/600 dilution (0.16 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
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All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell), whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 10 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
Myosin light chain-2 (MYL2, also called MLC-2) is an ~19kDa sarcomeric protein that belongs to the EF-hand calcium binding protein superfamily and exists as three major isoforms encoded by three distinct genes in mammalian striated muscle (PMID: 26074085). Although some cell lines are reported to be MYL2 positive, such as HeLa, 293T and MCF7 (PMID: 21487036, 21487036, 23093227), ab92721 only works in muscle and heart tissues according to our internal testing.
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All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution
Lane 1 :Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.1 µg
Lane 2 :Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 4 minutes -
All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution
Lane 1 :Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.1 µg
Lane 2 :Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 8 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab92721 has been referenced in 32 publications.
- Gong JE et al. Anti-Cancer Effects of α-Cubebenoate Derived from Schisandra chinensis in CT26 Colon Cancer Cells. Molecules 27:N/A (2022). PubMed: 35164001
- Gong W et al. CCL4-mediated targeting of spleen tyrosine kinase (Syk) inhibitor using nanoparticles alleviates inflammatory bowel disease. Clin Transl Med 11:e339 (2021). PubMed: 33634985
- Markandran K et al. Mn2+ -Phos-Tag Polyacrylamide for the Quantification of Protein Phosphorylation Levels. Curr Protoc 1:e221 (2021). PubMed: 34411463
- Kim JE et al. Laxative Effects of Phlorotannins Derived from Ecklonia cava on Loperamide-Induced Constipation in SD Rats. Molecules 26:N/A (2021). PubMed: 34885790
- Guo C et al. Bilobalide reversibly modulates blood-brain barrier permeability through promoting adenosine A1 receptor-mediated phosphorylation of actin-binding proteins. Biochem Biophys Res Commun 526:1077-1084 (2020). PubMed: 32312522