Key features and details
- Rabbit polyclonal to Myosin light chain 3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Myosin light chain 3 antibody
See all Myosin light chain 3 primary antibodies
DescriptionRabbit polyclonal to Myosin light chain 3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
Recombinant full length protein corresponding to Human Myosin light chain 3 aa 1-195.
MAPKKPEPKKDDAKAAPKAAPAPAPPPEPERPKEVEFDASKIKIEFTPEQ IEEFKEAFMLFDRTPKCEMKITYGQCGDVLRALGQNPTQAEVLRVLGKPR QEELNTKMMDFETFLPMLQHISKNKDTGTYEDFVEGLRVFDKEGNGTVMG AELRHVLATLGERLTEDEVEKLMAGQEDSNGCINYEAFVKHIMSS
Database link: NP_000249.1
- Myosin light chain 3-transfected 293T cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab172073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.|
FunctionRegulatory light chain of myosin. Does not bind calcium.
Involvement in diseaseDefects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
Sequence similaritiesContains 3 EF-hand domains.
modificationsThe N-terminus is blocked.
N-terminus is methylated by METTL11A/NTM1.
- Information by UniProt
- Cardiac myosin light chain 1 antibody
- CMH8 antibody
- CMLC1 antibody
All lanes : Anti-Myosin light chain 3 antibody (ab172073) at 1 µg/ml
Lane 1 : Myosin light chain 3-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Rabbit IgG (H+L) at 1/7500 dilution
Developed using the ECL technique.
Predicted band size: 22 kDa
ab172073 has not yet been referenced specifically in any publications.