Overview

  • Product name

    Anti-Myosin light chain 3 antibody [EPR4160]
    See all Myosin light chain 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR4160] to Myosin light chain 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Myosin light chain 3 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • human heart muscle lysate, human skeletal muscle lysate; human cardiac muscle tissue, human skeletal muscle tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Regulatory light chain of myosin. Does not bind calcium.
    • Involvement in disease

      Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
      Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
    • Sequence similarities

      Contains 3 EF-hand domains.
    • Post-translational
      modifications

      The N-terminus is blocked.
      N-terminus is methylated by METTL11A/NTM1.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cardiac myosin light chain 1 antibody
      • CMH8 antibody
      • CMLC1 antibody
      • ELC of myosin antibody
      • Essential light chain of myosin antibody
      • MLC1SB antibody
      • MLC1V antibody
      • MYL3 antibody
      • MYL3_HUMAN antibody
      • Myosin light chain 1 antibody
      • Myosin light chain 1 slow twitch muscle B/ventricular isoform antibody
      • Myosin light chain 1 slow, B antibody
      • Myosin light chain 3 antibody
      • Myosin, light chain 1, ventricular antibody
      • myosin, light chain 3, alkali; ventricular, skeletal, slow antibody
      • myosin, light polypeptide 3, alkali antibody
      • myosin, light polypeptide 3, alkali; ventricular, skeletal, slow antibody
      • OTTHUMP00000165922 antibody
      • Slow skeletal ventricular myosin alkali light chain 3 antibody
      • slow-twitch muscle B/ventricular isoform antibody
      • Ventricular/slow twitch myosin alkali light chain antibody
      • VLC1 antibody
      see all

    Images

    • Immunohistochemical staining of paraffin-embedded human cardiac muscle using ab108923 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical staining of paraffin-embedded human skeletal muscle tissue using ab108923 at a diltion of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-Myosin light chain 3 antibody [EPR4160] (ab108923) at 1/1000 dilution

      Lane 1 : human heart muscle lysate
      Lane 2 : human skeletal muscle lysate

      Lysates/proteins at 10 µg/ml per lane.

      Predicted band size: 22 kDa

    • ab108923 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108923 showing negative staining in Normal liver tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108923 showing negative staining in Normal tonsil tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108923 showing negative staining in Normal breast tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). ICC ; Goat . Read more (PubMed: 24699686) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108923.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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