RecombinantRabMAb

Recombinant Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4160] to Myosin light chain 3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free
    See all Myosin light chain 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4160] to Myosin light chain 3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Myosin light chain 3 aa 1-100. The exact sequence is proprietary.

  • General notes

    Ab247710 is the carrier-free version of ab108923. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247710 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Regulatory light chain of myosin. Does not bind calcium.

    • Involvement in disease

      Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
      Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.

    • Sequence similarities

      Contains 3 EF-hand domains.

    • Post-translational
      modifications

      The N-terminus is blocked.
      N-terminus is methylated by METTL11A/NTM1.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Cardiac myosin light chain 1 antibody
      • CMH8 antibody
      • CMLC1 antibody
      • ELC of myosin antibody
      • Essential light chain of myosin antibody
      • MLC1SB antibody
      • MLC1V antibody
      • MYL3 antibody
      • MYL3_HUMAN antibody
      • Myosin light chain 1 antibody
      • Myosin light chain 1 slow twitch muscle B/ventricular isoform antibody
      • Myosin light chain 1 slow, B antibody
      • Myosin light chain 3 antibody
      • Myosin, light chain 1, ventricular antibody
      • myosin, light chain 3, alkali; ventricular, skeletal, slow antibody
      • myosin, light polypeptide 3, alkali antibody
      • myosin, light polypeptide 3, alkali; ventricular, skeletal, slow antibody
      • OTTHUMP00000165922 antibody
      • Slow skeletal ventricular myosin alkali light chain 3 antibody
      • slow-twitch muscle B/ventricular isoform antibody
      • Ventricular/slow twitch myosin alkali light chain antibody
      • VLC1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)

      This data was developed using ab108923, the same antibody clone in a different buffer formulation.

      Immunohistochemical staining of paraffin-embedded human cardiac muscle using ab108923 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)

      This data was developed using ab108923, the same antibody clone in a different buffer formulation.

      Immunohistochemical staining of paraffin-embedded human skeletal muscle tissue using ab108923 at a diltion of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      Western blot - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      All lanes : Anti-Myosin light chain 3 antibody [EPR4160] (ab108923) at 1/1000 dilution

      Lane 1 : human heart muscle tissue lysate
      Lane 2 : human skeletal muscle tissue lysate

      Lysates/proteins at 10 µg/ml per lane.

      Predicted band size: 22 kDa



      This data was developed using ab108923, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      This data was developed using ab108923, the same antibody clone in a different buffer formulation.

      ab108923 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      This data was developed using ab108923, the same antibody clone in a different buffer formulation.

      ab108923 showing negative staining in Normal liver tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      This data was developed using ab108923, the same antibody clone in a different buffer formulation.

      ab108923 showing negative staining in Normal tonsil tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      This data was developed using ab108923, the same antibody clone in a different buffer formulation.

      ab108923 showing negative staining in Normal breast tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
      Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247710? Please let us know so that we can cite the reference in this datasheet.

    ab247710 has not yet been referenced specifically in any publications.

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