Recombinant Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free (ab247710)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Myosin light chain 3 antibody [EPR4160] - BSA and Azide free
See all Myosin light chain 3 primary antibodies -
Description
Rabbit monoclonal [EPR4160] to Myosin light chain 3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Myosin light chain 3 aa 1-100. The exact sequence is proprietary.
-
General notes
Ab247710 is the carrier-free version of ab108923. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247710 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4160 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
Target
-
Function
Regulatory light chain of myosin. Does not bind calcium. -
Involvement in disease
Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
Sequence similarities
Contains 3 EF-hand domains. -
Post-translational
modificationsThe N-terminus is blocked.
N-terminus is methylated by METTL11A/NTM1. - Information by UniProt
-
Database links
- Entrez Gene: 4634 Human
- Entrez Gene: 17897 Mouse
- Entrez Gene: 24585 Rat
- Omim: 160790 Human
- SwissProt: P08590 Human
- SwissProt: P09542 Mouse
- SwissProt: P16409 Rat
- Unigene: 517939 Human
see all -
Alternative names
- Cardiac myosin light chain 1 antibody
- CMH8 antibody
- CMLC1 antibody
see all
Datasheets and documents
References
ab247710 has not yet been referenced specifically in any publications.
