Overview

  • Product name

    Anti-Myosin light chain kinase/MLCK antibody
    See all Myosin light chain kinase/MLCK primary antibodies
  • Description

    Goat polyclonal to Myosin light chain kinase/MLCK
  • Host species

    Goat
  • Specificity

    ab130135 is expected to recognise all reported isoforms (NP_444253.3; NP_444254.3 ; NP_444255.3 and NP_444256.3).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Pig, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human Myosin light chain kinase/MLCK aa 1721-1734 (internal sequence) (Cysteine residue).
    Sequence:

    C-KDTKNMEAKKLSKD

  • Positive control

    • Human Colon and Lung lysates.
  • General notes

    Previously labelled as Myosin light chain kinase.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 211 kDa.

Target

  • Function

    Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
  • Tissue specificity

    Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 fibronectin type-III domain.
    Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    MLCK is probably down-regulated by phosphorylation.
    The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
  • Information by UniProt
  • Database links

  • Alternative names

    • deglutamylated form antibody
    • DKFZp686I10125 antibody
    • EC 2.7.11.18 antibody
    • FLJ12216 antibody
    • Kinase related protein antibody
    • Kinase-related protein antibody
    • KRP antibody
    • MLCK antibody
    • MLCK1 antibody
    • MLCK108 antibody
    • MLCK210 antibody
    • MSTP083 antibody
    • MYLK antibody
    • MYLK_HUMAN antibody
    • MYLK1 antibody
    • Myosin light chain kinase antibody
    • Myosin light polypeptide kinase antibody
    • OTTHUMP00000180642 antibody
    • OTTHUMP00000180643 antibody
    • smMLCK antibody
    • smooth muscle antibody
    • Smooth muscle myosin light chain kinase antibody
    • Telokin antibody
    see all

Images

  • Anti-Myosin light chain kinase/MLCK antibody (ab130135) at 0.03 µg/ml (incubated for 1 hour) + Human Colon lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 211 kDa

References

This product has been referenced in:

  • Zhong X  et al. Reverse mode of sodium/calcium exchanger subtype 1 contributes to detrusor overactivity in rats with partial bladder outflow obstruction. Am J Transl Res 10:806-815 (2018). IHC ; Rat . Read more (PubMed: 29636870) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 21 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 21 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 20 2015

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