Key features and details
- Rabbit polyclonal to Myosin light chain kinase/MLCK
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Myosin light chain kinase/MLCK antibody
See all Myosin light chain kinase/MLCK primary antibodies
DescriptionRabbit polyclonal to Myosin light chain kinase/MLCK
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Rabbit, Cow
Recombinant fragment corresponding to Human Myosin light chain kinase/MLCK aa 1661-1877. (N-terminal His-tag and GST-tag; Expressed in E.coli).
FMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDC TQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSS MAMISGLSGRKSSTGSPTSPLNAEKLESEEDVSQAFLEAVAEEKPHVKPY FSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYD EDGNCSLIISDVCGDDD
Database link: Q15746
- IHC-P: Human esophagus cancer and kidney tissues. WB: Human lung lysate; Rat prostate gland and bladder lysates; Recombinant human Myosin light chain kinase/MLCK protein.
Previously labelled as Myosin light chain kinase.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232949 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 211 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionCalcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
Tissue specificitySmooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 fibronectin type-III domain.
Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsMLCK is probably down-regulated by phosphorylation.
The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
- Information by UniProt
- deglutamylated form antibody
- DKFZp686I10125 antibody
- EC 220.127.116.11 antibody
Paraffin-embedded human esophagus cancer tissue stained for Myosin light chain kinase/MLCK antibody using ab232949 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for Myosin light chain kinase/MLCK antibody using ab232949 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Myosin light chain kinase/MLCK antibody (ab232949) at 2 µg/ml + Recombinant human Myosin light chain kinase/MLCK protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 211 kDa
All lanes : Anti-Myosin light chain kinase/MLCK antibody (ab232949) at 2 µg/ml
Lane 1 : Human lung lysate
Lane 2 : Rat prostate gland lysate
Lane 3 : Rat bladder lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 211 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232949 has not yet been referenced specifically in any publications.