Overview

  • Product name

    Anti-Myosin light chain kinase/MLCK antibody
    See all Myosin light chain kinase/MLCK primary antibodies
  • Description

    Rabbit polyclonal to Myosin light chain kinase/MLCK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Myosin light chain kinase/MLCK aa 290-398.
    Database link: Q15746

  • Positive control

    • IHC-P: Human smooth muscle tissue. ICC/IF: HepG2 cells.
  • General notes

    Previously labelled as Myosin light chain kinase.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.

Target

  • Function

    Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
  • Tissue specificity

    Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 fibronectin type-III domain.
    Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    MLCK is probably down-regulated by phosphorylation.
    The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
  • Information by UniProt
  • Database links

  • Alternative names

    • deglutamylated form antibody
    • DKFZp686I10125 antibody
    • EC 2.7.11.18 antibody
    • FLJ12216 antibody
    • Kinase related protein antibody
    • Kinase-related protein antibody
    • KRP antibody
    • MLCK antibody
    • MLCK1 antibody
    • MLCK108 antibody
    • MLCK210 antibody
    • MSTP083 antibody
    • MYLK antibody
    • MYLK_HUMAN antibody
    • MYLK1 antibody
    • Myosin light chain kinase antibody
    • Myosin light polypeptide kinase antibody
    • OTTHUMP00000180642 antibody
    • OTTHUMP00000180643 antibody
    • smMLCK antibody
    • smooth muscle antibody
    • Smooth muscle myosin light chain kinase antibody
    • Telokin antibody
    see all

Images

  • Paraffin-embedded human smooth muscle tissue stained for Myosin light chain kinase/MLCK using ab236299 at 1/100 dilution in immunohistochemical analysis.

  • HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Myosin light chain kinase/MLCK (green) using ab236299 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

References

ab236299 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab236299.
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