Key features and details
- Rabbit polyclonal to Myosin light chain kinase/MLCK
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Myosin light chain kinase/MLCK antibody
See all Myosin light chain kinase/MLCK primary antibodies
DescriptionRabbit polyclonal to Myosin light chain kinase/MLCK
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Myosin light chain kinase/MLCK aa 290-398.
Database link: Q15746
- IHC-P: Human smooth muscle tissue. ICC/IF: HepG2 cells.
Previously labelled as Myosin light chain kinase.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
FunctionCalcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
Tissue specificitySmooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 fibronectin type-III domain.
Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsMLCK is probably down-regulated by phosphorylation.
The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
- Information by UniProt
- deglutamylated form antibody
- DKFZp686I10125 antibody
- EC 126.96.36.199 antibody
Paraffin-embedded human smooth muscle tissue stained for Myosin light chain kinase/MLCK using ab236299 at 1/100 dilution in immunohistochemical analysis.
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Myosin light chain kinase/MLCK (green) using ab236299 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236299 has not yet been referenced specifically in any publications.